仿生型低温保护剂研究进展  

Research Progress of Biomimetic Cryoprotectants

作　　者：陈曦 李维杰[1,2,3] 刘宝林 CHEN Xi;LI Weijie;LIU Baolin(Institute of Biothermal Science and Technology,University of Shanghai for Science and Technology,Shanghai 200093,China;Co-innovation Center for Energy Therapy of Tumors,Shanghai 200093,China;Shanghai Technical Service Platform for Cryopreservation of Biological Resources,Shanghai 200093,China)

机构地区：[1]上海理工大学生物系统热科学研究所,上海200093 [2]上海市肿瘤能量治疗技术与器械协同创新中心,上海200093 [3]上海市生物资源低温保存技术服务平台,上海200093

出　　处：《制冷技术》2022年第3期80-85,共6页Chinese Journal of Refrigeration Technology

基　　金：上海市生物资源低温保存技术服务平台。

摘　　要：低温保护剂是低温保存技术的重要组成部分。本文综述了仿生型低温保护剂的发展进程,主要包括低温冷冻损伤机制,传统保护剂种类、仿生型保护剂机制研究、在细胞、组织以及器官中的应用。结果表明:仿生型低温保护剂能够有效抑制冰晶生长和重结晶现象,减少细胞损伤,从而提高细胞存活率。此类保护剂具有较高的生物相容性且无毒,可以降低二甲基亚砜等有毒试剂的用量,是一种很有潜力的低温保护剂。Cryoprotectant is an important part of cryopreservation technology.The development process of biomimetic cryoprotectants,mainly including the mechanism of cryoinjury,the types of traditional protectants,the mechanism of biomimetic protectants,and their applications in cells,tissues,and organs are reviewed in this paper.The results show that the biomimetic cryoprotectant can effectively inhibit the growth and recrystallization of ice crystals,reduce cell damage,and improve cell survival rate.This kind of protective agent has high biocompatibility and is non-toxic.It can reduce the dosage of toxic reagents such as dimethyl sulfoxide.It is a potential low-temperature protective agent.

关 键 词：仿生型低温保护剂 冷冻损伤机制 生物相容性 

分 类 号：TB611[一般工业技术—制冷工程] R318.52[医药卫生—生物医学工程]