嗜酸浸矿细菌的定向富集筛选及对多金属硫化矿尾矿的生物浸出特征  

Directional Enrichment and Screening of Acidophilic Leaching Bacteria and Bioleaching with Polymetallic Sulphide Tailings

作　　者：聂圆圆 王芷晴 谭晓桐 姜海强 安龙[1,2] 赵鑫[1] NIE Yuanyuan;WANG Zhiqing;TAN Xiaotong;JIANG Haiqiang;AN Long;ZHAO Xin(School of Resources and Civil Engineering,Northeastern University,Shenyang 110819,China;Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines,Shenyang 110819,China)

机构地区：[1]东北大学资源与土木工程学院,辽宁沈阳110819 [2]深部金属矿山安全开采教育部重点实验室,辽宁沈阳110819

出　　处：《金属矿山》2022年第8期125-130,共6页Metal Mine

基　　金：“十三五”国家重点研发计划项目(编号:2019YFC1907204);辽宁省重点研发计划项目(编号:2019020104-JH2/103)。

摘　　要：以山东省某金属矿的酸性矿井水为接种物,定向富集筛选获得1株具有嗜酸、氧化Fe^(2+)能力的浸矿细菌,编号WT2020。经生理生化和16S rDNA基因测序分析,鉴定为嗜酸氧化亚铁硫杆菌,与模式菌株Acidithiobacillus ferrooxidans strain ATCC 23270^(T)(NR074193)的同源性为100%。探究菌株WT2020的铁氧化活性和金属耐受性,确定其最佳接种量和可耐受矿浆浓度分别为15%和100 g/L。利用菌株WT2020对金都矿业的多金属硫化矿尾矿进行浸出试验,在最优接种量和矿浆浓度条件下浸矿28 d,Cu^(2+)和Zn^(2+)浸出率分别达到55.23%和72.09%。A bioleaching functional strain WT2020 with the capability of acidophilic and Fe^(2+)oxidation was directional enriched and isolated from acid mine drainage of a metallic mine in Shandong.Basing on the physiological and biochemical features,and the 16S rDNA gene sequencing result,it was identified belonging to Acidithiobacillus ferrooxidans with a 100%similarity to the type strain A.ferrooxidans ATCC 23270^(T)(NR074193).The iron oxidation activity and metal tolerance of strain WT2020 were verified,the optimal inoculum of 15%and tolerable pulp density of 100 g/L were obtained.Strain WT2020 was applied in the bioleaching from a polymetallic sulphide tailings of Jindu Mining,in which Cu^(2+)and Zn^(2+)leaching rates of 55.23%and 72.09%were obtained under the optimal leaching condition for 28 d.

关 键 词：生物浸矿 ACIDITHIOBACILLUS 铁氧化活性 金属耐受性 多金属硫化矿尾矿 

分 类 号：TD926.5[矿业工程—选矿]