小豆Dirigent基因家族鉴定及锈菌侵染对不同成员表达的影响  

Dirigent gene family identification and expression profiling of Vigna angularis responsive to Uromyces vignae infection

作　　者：柯希望[1] 苑梦琦 徐晓丹 殷丽华[1] 郭永霞[1] 左豫虎[1] KE Xi-Wang;YUAN Meng-Qi;XU Xiao-Dan;YIN Li-Hua;GUO Yong-Xia;ZUO Yu-Hu(College of Agriculture,Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology Eco-logical Control/National Coarse Cereals Engineering Research Center,Daqing 163319,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学农学院/黑龙江省作物有害生物互作生物学及生态防控重点实验室/国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《作物学报》2022年第11期2774-2785,共12页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32102173);黑龙江省自然科学基金项目(YQ2020C034);黑龙江八一农垦大学研究生创新项目(YJSCX2021-Y50)资助。

摘　　要：为明确Dirigent (DIR)基因在小豆抗锈病中的作用,采用生物信息学方法,对小豆DIR基因家族(VaDIRs)进行了全基因组鉴定,结果发现小豆中共有33个DIR基因,其中29个成员分别定位在8条染色体上,系统进化分析表明,VaDIRs包括DIR-a、DIR-b/d、DIR-e和DIR-f四个亚家族,对VaDIRs成员启动子区顺式作用元件的分析发现,VaDIRs启动子区均包含激素、病原体应答等元件。对小豆抗锈病品种接种后不同时间的转录组数据分析发现,差异表达基因中共有17个VaDIRs成员,其中6个于接种后24 h显著上调,2个于接种后24 h和48 h均高量表达。进一步应用qRT-PCR技术对上述8个基因在抗、感品种中应答锈菌侵染的表达分析表明,高抗品种中VaDIR14、VaDIR16和VaDIR33的表达量在锈菌侵染不同阶段均显著高于感病品种。上述结果表明,VaDIRs家族成员可作为正调节因子参与小豆对锈病的抗性。To clarify the role of Dirigent(DIR)gene family of adzuki bean(Vigna angularis)in response to Uromyces vignae infection,genome-wide identification of the DIR family genes of V.angularis(VaDIRs)was conducted.A total of 33 VaDIRs in the V.angularis genome were identified,among which 29 members were located on 8 chromosomes,respectively.Phylogenetic analysis showed that VaDIRs consisted of 4 subfamilies(DIR-a,DIR-b/d,DIR-e,and DIR-f).Cis-acting elements analysis of the promoter of VaDIRs indicated that all members of VaDIRs contained hormones and pathogen responsive elements.Transcriptomic data of the rust resistant cultivar at different times after inoculation revealed that 17 VaDIRs members were differentially ex-pressed,among which 6 VaDIRs were significantly upregulated at 24 hours post inoculation(hpi),and 2 members were highly expressed both at 24 and 48 hpi.The relative expression of the genes mentioned above were further confirmed in different resistant cultivars responsive to U.vignae infection by qRT-PCR.Results demonstrated that VaDIR14,VaDIR16,and VaDIR33 in re-sistant cultivar were significantly induced than those in the susceptible cultivar during the fungal infection.In short,our results indicated that members of VaDIRs could be used as positive regulators in resistant cultivar to prevent U.vignae infection.

关 键 词：小豆(Vigna angularis) 小豆锈病 豇豆单胞锈菌(Uromyces vignae) DIR基因 基因家族 

分 类 号：S435.21[农业科学—农业昆虫与害虫防治]