油菜F-box-LRR基因全基因组鉴定与核盘菌诱导应答分析  

Genome-wide characterization of F-box-LRR genes and their transcript response to Sclerotinia sclerotiorum induction in Brassica napus

作　　者：左蓉 吴姗 刘杰 胡鸣 程晓晖[1] 刘越英[1] 白泽涛[1] 刘胜毅[1] ZUO Rong;WU Shan;LIU Jie;HU Ming;CHENG Xiao-hui;LIU Yue-ying;BAI Ze-tao;LIU Sheng-yi(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所,农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2022年第3期503-514,共12页Chinese Journal of Oil Crop Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(Y2020YJ03);国家油菜产业技术体系(CARS-12)。

摘　　要：由核盘菌引起的菌核病是一种重要的真菌性病害,筛选菌核病抗病基因对抗病育种具有重要意义。F-box基因多参与植物抗逆反应,LRR作为抗病基因的重要结构域,在植物抗病防卫中起着重要的作用。本研究通过生物信息学方法在甘蓝型油菜基因组中对F-box-LRR基因进行了全基因组鉴定,基于已发表的中双11组织表达数据以及油菜不同品种中油821(抗病)和Westar(感病)接种核盘菌前后的转录组数据,对可能响应核盘菌诱导的BnF-box-LRR基因进行筛选,并结合荧光定量PCR进行验证。共鉴定到161个BnF-box-LRR基因,从系统进化树上可分为4个亚类(FBXLRR1,FBXLRR2,FBXLRR3和FBXLRR4),其中第四亚类FBXLRR4在蛋白保守序列分布以及基因结构方面,与其它三个亚类具有较大差异,且与拟南芥参与植物抗逆的同源基因聚为一类,因此推测该分支可能主要参与植物胁迫响应。表达分析表明FBXLRR4家族基因在根和叶中有较高的表达水平,且在核盘菌诱导后具有明显的表达变化,暗示这些基因可能参与油菜菌核病抗性功能。Sclerotinia stem rot caused by Sclerotinia sclerotiorum is an important fungi disease in oil crops especially in Brassica napus.For plant breeding,screening of Sclerotinia resistance genes is important.Most F-box genes participated in plant defense response,and LRR regarded as the important domain of resistant genes.Thereby,F-box genes were investigated in this study.Genome-wide identification of F-box-LRR gene in B.napus was conducted by bioinformatics method.Based on published data of tissue expression in Zhongshuang 11 and transcriptome data of inoculation fungi on leaves of susceptible Westar and resistant Zhongyou 821,corresponding Bn-F box-LRR genes were identified and then verified by qRT-PCR.161 BnF-box-LRR genes were found and classified into 4 subgroups(FBXLRR1 to FBXLRR4).The fourth subgroup,FBXLRR4,was significantly different from the other three in the conserved protein distribution and gene structure.It was clustered into the same clade with the homologous genes which involved in plant stress resistance in Arabidopsis thaliana.Therefore,it is speculated that this subgroup might be mainly involved in plant stress response.Based on transcriptome data,it was found that most FBXLRR4 genes were highly expressed in root and leaves,and showed significant expression alternation after S.sclerotiorum induction,indicating these genes potentially in Sclerotinia disease resistant.

关 键 词：甘蓝型油菜 F-box-LRR基因家族 菌核病 基因表达 

分 类 号：Q789[生物学—分子生物学]