Efficient generation of targeted point mutations in the Brassica oleracea var.botrytis genome via a modified CRISPR/Cas9 system  

作　　者：Guixiang Wang Mei Zong Di Liu Yage Wu Shouwei Tian Shuo Han Ning Guo Mengmeng Duan Liming Miao Fan Liu 

机构地区：[1]Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China

出　　处：《Horticultural Plant Journal》2022年第4期527-530,共4页园艺学报（英文版）

基　　金：partly funded by the project of technology innovation ability from Beijing Academy of Agriculture and Forestry Sciences (Grant Nos. KJCX20200401, KJCX20200205 and KJCX20200113);the Natural Science Foundation of China (Grant No. 31972401)

摘　　要：In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants.For ALS gene,a C-to-T conversion in the Pro182 codon(CCT)can alter the encoded amino acid,likely resulting in herbicide resistance,and a C-to-T mutation in the Leu133 codon(CTT)in the CENH3 gene may produce a haploid inducer.Results indicated that the transformation efficiency was 1.8%–4.5%and the mutation efficiencies for the ALS and CENH3 genes were approximately 22%and 87%,respectively.The ALS mutant cauliflower showed strong herbicide resistance,with possible immediate implications for broadleaf weed control in cauliflower fields.

关 键 词：CAULIFLOWER Targeted point mutations Base-editing CRISPR/Cas9 ALS CENH3 

分 类 号：S635.3[农业科学—蔬菜学]