一株海南菌草“绿洲一号”内生细菌的分离鉴定及其生物学特性研究  

Isolation,Identification and Biological Characteristics of An Endophytic Bacterium from Juncao “Oasis No.1” in Hainan

作　　者：李望豪 周瑞鹏 许钰滢 赵琪琪 刘娟 王荣波 叶文雨[1,2] LI Wanghao;ZHOU Ruipeng;XU Yuying;ZHAO Qiqi;LIU Juan;WANG Rongbo;YE Wenyu(National Engineering Research Center of Juncao Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Life Sciences,Fujian Agriculture and Forestry University/Fujian University Key Laboratory for Plant-Microbe Interaction,Fuzhou 350002,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou 350013,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学生命科学学院/植物与微生物相互作用福建省高校重点实验室,福建福州350002 [3]福建农林大学园艺学院,福建福州350002 [4]福建省作物有害生物监测与治理重点实验室,福建福州350013

出　　处：《广东农业科学》2022年第7期81-88,共8页Guangdong Agricultural Sciences

基　　金：福建农林大学科技创新专项基金(KFA19137A);福建省作物有害生物监测与治理重点实验室开放课题(MIMCP-202002);福建省科技重大专项(2021NZ029009);福建省引导性科技计划项目(2022N0010)。

摘　　要：【目的】探讨1株从海南菌草“绿洲一号”叶片中分离鉴定的内生细菌HLY3-2的促生、拮抗病原真菌等特性,为植物病原真菌的生物防治及植物促生机理研究提供基础理论。【方法】以海南菌草“绿洲一号”叶片为材料,运用稀释涂布平板法分离内生细菌HLY3-2。通过形态学鉴定、16SrDNA序列测定及同源性分析,构建系统发育树确定该菌属种。采用平板对峙法并利用各种生理生化培养基、促生性能培养基研究其生物学特性。【结果】通过内生细菌形态学、生理生化特性和16SrDNA序列测定,HLY3-2被鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。拮抗病原真菌筛选试验结果表明,内生细菌HLY3-2对稻瘟病菌(Magnaporthe oryzae,Guy11)、香蕉枯萎病菌4号(Foctropical race4,Foc4)均有一定的抑菌作用,抑菌率分别为18.45%和30.65%。生理生化特性分析结果表明,该内生细菌不具有利用柠檬酸盐、纤维素与淀粉的能力;在生长过程中不产生酯类物质,也无法将明胶水解,但其在生长过程中具有产生蛋白酶和过氧化氢酶的能力。促生性能分析结果表明,该内生细菌具溶解无机磷和有机磷、降解硅酸盐的能力,不具有产铁载体潜力,但具有很强的产IAA能力。【结论】内生细菌HLY3-2在促进植物生长和防治病害中有一定作用。【Objective】The study aimed to explore the growth-promoting properties and antagonism of pathogenic fungi of an endophytic bacterium HLY3-2 isolated and identified from the leaves of Hainan Juncao“Oasis No.1”,with a view to providing basic theory for the biological control of plant pathogenic fungi and the study of plant probiotic mechanism.【Method】The endophytic bacterium HLY3-2 was isolated from the leaves of Hainan Juncao “Oasis No.1” by using the dilution plating method.Through morphological identification,16SrDNA sequencing and homology analysis,a phylogenetic tree was constructed to identify the genus and species of this strain.The biological characteristics were explored by using the plate antagonism method and various physiology and biochemistry culture medium and growth-promoting property culture medium.【Result】HLY3-2 was identified as a strain of Bacillus thuringiensis through the endophytic bacterium morphology,physiology and biochemistry characteristics and 16SrDNA sequencing.The results of antagonism of pathogenic fungi showed that the strain HLY3-2 had some inhibition effect on Magnaporthe oryzae and Foctropical race4 with the bacteriostasis rates of 18.45% and 30.65% respectively.Then,according to results of the analysis on physiology and biochemistry characteristics,the strain did not have the ability of utilizing citrate,cellulose and starch.And it could not produce ester substances and hydrolyze gelatin during growth,but it had the ability of producing proteases and catalase during its growth and metabolism process.The results of growth-promoting property analysis showed that the strain had the ability to dissolve inorganic phosphorus and organophosphorus and degrade silicates,and could not produce iron carriers,but had strong IAA-producing ability.【Conclusion】The experiment results show that the endophytic bacterium HLY3-2 has a role in promoting plant growth and controlling disease.

关 键 词：菌草 内生细菌 生物学特性 拮抗活性 促生潜力 

分 类 号：S482.292[农业科学—农药学]