北方强冬性甘蓝型冬油菜生长锥与抗寒性的关系研究  

Relationship between height of growth point and cold resistance in strong winter rape(Brassica napus L.)in Northern China

作　　者：李学才[1] 金姣姣 马骊[1] 武军艳[1] 陈其鲜 曾瑞 曾秀存[4] 崔小茹 孙万仓[1] LI Xue-cai;JIN Jiao-jiao;MA Li;WU Jun-yan;CHEN Qi-xian;ZENG Rui;ZENG Xiu-cun;CUI Xiao-ru;SUN Wan-cang(College of Agronomy,Gansu Agricultural University/Gansu Provincial Key Laboratory of Arid Land Crop Sciences/Gansu Research Center of Rapeseed Engineering and Technology/Key Laboratory of Crop Genetics Improvement and Germplasm Enhancement of Gansu Province,Lanzhou 730070,China;Gansu Province Dryland Winter Wheat Germplasm Innovation and Application Engineering Research Center,Provincial 2011 Collaborative Innovation Center,College of Agriculture and Forestry,Longdong University,Qingyang 745000,China;Gansu Agricultural technology Extension Station,Lanzhou 730070,China;Hexi University,Zhangye 734000,China)

机构地区：[1]甘肃农业大学农学院/甘肃省共建干旱生境作物学国家重点实验室/甘肃省油菜工程技术研究中心/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃省旱地冬小麦种质创新与应用工程研究中心/省级2011协同创新中心/陇东学院农林科技学院,甘肃庆阳745000 [3]甘肃省农业技术推广总站,甘肃兰州730070 [4]河西学院,甘肃张掖734000

出　　处：《中国油料作物学报》2022年第4期739-750,共12页Chinese Journal of Oil Crop Sciences

基　　金：陇东学院青年科技创新项目(XYZK2106);2021年现代丝路寒旱农业科技支撑项目(GSLK-2021-12);国家现代农业产业技术体系(CARS-12)。

摘　　要：为探究北方强冬性甘蓝型冬油菜(Brassica napus L.)生长锥低温响应特性及其与抗寒性的关系,以冬性不同的甘蓝型冬油菜为试验材料,通过体式显微镜解剖、石蜡切片、扫描电镜等方法观察生长锥形态,通过测定半致死温度(LT_(50))鉴定不同品种的抗寒性,并对STM等生长锥调控关键基因进行克隆和表达分析。结果表明,冬前低温处理后,强冬性抗寒品种VHTSG10与VHTS309-4苗期的生长锥表面光滑,相对高度较低,弱冬性材料VH天油2288和VH新油23号苗期的生长锥表面有小突起组织,相对高度较高;不同品种间LT_(50)与生长锥相对高度极显著正相关;调控苗期生长锥分化的关键基因BnSTM,其相对表达量与生长锥相对高度显著正相关,而BnCUC2基因相对表达量与生长锥相对高度极显著负相关;BnSTM基因存在1149 bp的完整开放阅读框,编码382个氨基酸,包含KNOX1、KNOX2、ELK和Homeobox_KN 4个保守结构域,与白菜型油菜(Brassica rapa L.)的亲缘关系较近,蛋白序列相似性为98.69%,亚细胞定位分析表明BnSTM蛋白在细胞核表达,参与茎尖分生组织的生长发育,与预测结果一致。因此,冬性越强的甘蓝型冬油菜,苗期生长锥相对高度越低,抗寒性越强,而冬性弱的品种苗期生长锥相对高度越高,抗寒性越差。In order to explore the response characteristics of growth point to low temperature,and to understand its relationship with cold resistance,growth point morphology of 4 rapeseed(Brassica napus L.)materials(cold resistant VHTSG10 and VHTS309-4;cold susceptive VH Tianyou 2288 and VH Xinyou 23)was observed by microscopy,paraffin section and scanning electron microscope.Cold resistance of the materials was identified by measuring the semi-lethal temperature(LT_(50)).Their growth point regulation genes were cloned.Morphology results showed that,under cold treatment before winter,growth points at seedling of VHTSG10 and VHTS309-4 had smooth surface and lower relative height,while those of VH Tianyu 2288 and VH Xinyou 23 had small protuberant structures and higher relative height.LT_(50) was positively correlated with the relative height of growth point.Relative expression of BnSTM was significantly positively correlated with the relative height of growth points,while relative expression of BnCUC2 was significantly negatively correlated with the relative height of seedling growth points.BnSTM gene has a complete open reading frame of 1149 bp and encodes 382 amino acids,including KNOX1,KNOX2,ELK and Homeobox_KN domains.BnSTM was closely related to B.rapa,and the protein sequence similarity was 98.69%.Subcellular localization analysis showed that BnSTM protein was found in nucleus and involved in growth and development of shoot apical meristem.Thus could be concluded that,the stronger the wintering,the lower the relative height of seedling growth point,and the stronger the cold resistance.

关 键 词：甘蓝型冬油菜 强冬性 生长锥 半致死温度 

分 类 号：S565.4[农业科学—作物学]