甘蓝型油菜子叶黄化致死突变体ytl的表型分析和基因定位  

Phenotypic identification and gene mapping of cotyledon yellowing lethality mutant ytl in Brassica napus

作　　者：邬瑜锦 徐凯 宋居荣 赵伦 文静[1] 易斌[1] 马朝芝[1] 沈金雄[1] 傅廷栋[1] 涂金星[1] WU Yu-jin;XU Kai;SONG Ju-rong;ZHAO Lun;WEN Jing;YI Bin;MA Chao-zhi;SHEN Jin-xiong;FU Ting-dong;TU Jin-xing(College of Plant Science and Technology,National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学植物科学技术学院/作物遗传改良国家重点实验室,湖北武汉430070

出　　处：《中国油料作物学报》2022年第4期762-769,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2016YFD0100305)。

摘　　要：甘蓝型油菜子叶黄化致死直接影响油菜出苗率和成苗率,深入研究子叶黄化致死的分子机制可为探究植物生理相关的基础研究提供便利。本文报道了甘蓝型油菜子叶黄化致死突变体ytl(yellow to lethal)的基因定位及候选基因预测结果。该突变体来自恢复系轮回选择群体的自交后代株系,发芽出土后子叶一直处于黄化状态,播种9~15 d后死亡。与野生型相比,突变体ytl的叶绿素、叶黄素含量显著降低。透射电镜观察显示,突变体叶绿体发育仍处于质体阶段,类囊体基粒片层模糊。遗传分析表明,该突变体由一对隐性核基因控制。利用油菜60K SNP芯片结合分子标记技术将该基因定位于C09染色体的标记SSR-140和标记PBZIN-1之间198 kb的物理区间。该研究为进一步克隆基因BnaC09.YTL及后续的功能研究奠定了基础。Cotyledon yellowing lethality directly affects seed emergence and seedling rate in Brassica napus.It is convenient to investigate the molecular mechanism of cotyledon yellowing lethal mutants for the basic research on plant physiology.In this paper,we reported the results of gene localization and candidate gene prediction related to the mutant ytl(yellow to lethal)in B.napus.The mutant isolated from the progenies of the restorer recurrent selection population did not return to green after germination,and the cotyledons remained in a state of yellowing and died in 9-15 days after sowing.The chlorophyll and lutein contents of the ytl mutant were significantly reduced compared with the wild-type plants.Transmission electron microscopic observations showed that the chloroplasts of the mutant still stopped at the plastid stage and the basal lamellae of the cystoid were blurred.The results of genetic analysis indicated that the cotyledon yellowing trait of the mutant was controlled by a recessive nuclear gene.The candidate gene was located between the marker SSR-140 and PBZIN-1 on chromosome C09 using B.napus 60K Illumina Infinium SNP microarray combined with molecular marker analysis,corresponding to a physical distance of 198 kb.This study laid the foundation for further cloning of the candidate gene BnaC09.YTL and subsequent functional researches.

关 键 词：甘蓝型油菜 子叶黄化致死 分子标记 基因定位 

分 类 号：S565.4[农业科学—作物学]