通过关联分析鉴定与花生脂肪酸含量相关分子标记  

Identification of markers stably associated with different fatty acid content in peanut through association analysis

作　　者：吴贝[1] 刘念[1] 黄莉[1] 罗怀勇[1] 周小静[1] 陈伟刚[1] 郭建斌[1] 淮东欣[1] 夏友霖[2] 雷永[1] 廖伯寿[1] 姜慧芳[1] WU Bei;LIU Nian;HUANG Li;LUO Huai-yong;ZHOU Xiao-jing;CHEN Wei-gang;GUO Jian-bin;HUAI Dong-xin;XIA You-lin;LEI Yong;LIAO Bo-shou;JIANG Hui-fang(Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Nanchong Academy of Agricultural Sciences,Nanchong 637000,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]四川省南充市农业科学研究所,四川南充637000

出　　处：《中国油料作物学报》2022年第4期818-825,共8页Chinese Journal of Oil Crop Sciences

基　　金：广东省重点领域研发计划(2020B020219003);国家自然科学基金(31801403,31871666);农作物种质资源保护项目(2017NWB033);国家农作物种质资源共享服务平台(NICGR2017-36);国家现代农业产业技术体系建设专项(CARS-13-花生种质资源评价)。

摘　　要：油酸、亚油酸和棕榈酸是花生油脂中最主要的3种脂肪酸,其含量是影响花生油脂品质的重要因素。提高油酸含量并降低亚油酸和棕榈酸含量是花生品质性状改良的重要方向之一。本研究利用292份中国花生种质资源材料及583个SSR标记基因型数据对四个环境下不同脂肪酸含量进行关联分析,分别检测到与油酸、亚油酸和棕榈酸含量稳定关联标记14,14和9个,其中8个标记同时与上述3种脂肪酸含量稳定关联,分布在A02、A03、A08和A09染色体上。AHGS2050-226bp和AHGS3647-253bp是两个新关联标记的优异等位位点,在花生微微核心种质中证实,AHGS2050-226bp可提高油酸(9.99%~11.26%)并降低亚油酸(8.04%~9.31%)和棕榈酸含量(1.86%~1.97%),AHGS3647-253bp可提高油酸(9.79%~10.44%)并降低亚油酸(8.09%~8.62%)和棕榈酸含量(1.81%~1.95%)。本研究鉴定的多环境稳定关联标记AHGS2050和AHGS3647具有辅助选择高油酸且低亚油酸和低棕榈酸品种的潜在应用价值。The composition of three major fatty acids,oleic,linoleic and palmitic acids together determine peanut oil quality.Increasing oleic acid content and decreasing linoleic acid and palmitic acid content is one of the important projects for peanut quality improvement.In this study,292 peanut germplasm resources from China and their 583 SSR markers were used to analyze the association between fatty acid composition and molecular markers in four environments.There were 14,14,and 9 stable associated markers repeatedly detected in multiple environments for oleic acid,linoleic acid,and palmitic acid content,respectively.Among them,8 stable markers were simultaneously associated with the three fatty acids,distributing on chromosomes A02,A03,A08 and A09.AHGS2050-226bp and AHGS3647-253bp were two favorable alleles of novel associated markers for three major fatty acids.In peanut mini-core germplasm,AHGS2050-226bp was verified to stably increase oleic acid content(9.79%-11.26%),and reduce the contents of linoleic acid(8.04%-9.31%)and palmitic acid(1.81%-1.97%).AHGS3647-253bp could gain oleic acid content(9.79%-11.26%),and decrease the contents of linoleic acid(8.04%-9.31%)and palmitic acid(1.81%-1.97%)in mini-core germplasm.Stable associated markers(AHGS2050 and AHGS3647)have potential value in assisting variety selection of high oleic acid and low linoleic acid and palmitic acid in peanut breeding.

关 键 词：花生 棕榈酸 油酸 亚油酸 关联分析 

分 类 号：S565.2[农业科学—作物学]