基于声表面波气相色谱仪的唾液中苯丙胺和甲基苯丙胺快速检测  

Rapid determination of amphetamine and methamphetamine in saliva by using surface acoustic wave gas chromatography

作　　者：朱宏伟[1] 孙林 张凯[2] 陆艳艳[1] 孟祥宇 刘久玲[1] 何世堂[1] ZHU Hong-wei;SUN Lin;ZHANG Kai;LU Yan-yan;MENG Xiang-yu;LIU Jiu-ling;HE Shi-tang(Institute of Acoustics,Chinese Academy of Sciences,Beijing 100190,China;Anti-Drug Corps of Tianjin Public Security Bureau,Tianjin 300240,China;Beijing Vocational College of Agriculture,Beijing 100080,China)

机构地区：[1]中国科学院声学研究所,北京100190 [2]天津市公安局禁毒总队,天津300240 [3]北京农业职业学院,北京100080

出　　处：《化学研究与应用》2022年第8期1939-1944,共6页Chemical Research and Application

基　　金：中国科学院项目(ZDRW-XH-2019-4/1)资助。

摘　　要：建立了同时检测唾液中苯丙胺和甲基苯丙胺的快速前处理和顶空-声表面波气相色谱方法。对前处理方法中的NaOH使用量、振荡速度以及振荡时间等参数进行了考察。结果显示,最佳前处理条件为NaOH使用量2.5 g、振荡速度800 r·min^(-1)以及振荡时间4 min。最佳条件下,声表面波气相色谱仪测定苯丙胺和甲基苯丙胺的检出限分别为0.14μg·L^(-1)和1.15μg·L^(-1),线性范围为5~75μg·L^(-1),线性相关系数分别为0.995和0.994。在12.5μg·L^(-1)和25μg·L^(-1)添加水平时,回收率范围为89.6%~106.9%,相对标准偏差为4.3%~8.1%(n=6)。本方法采用快速前处理结合声表面波气相色谱仪,操作简单,检测灵敏度高、速度快,适用于公安机关毒驾现场检测初筛工作。A method of rapid pretreatment of saliva and the simultaneous determination of amphetamine and methamphetamine in saliva by using surface acoustic wave gas chromatography(GC-SAW)was developed.The influence factors such as NaOH dosage,oscillation speed and oscillation time in the pretreatment method were investigated.The results showed that the optimum pretreatment conditions were NaOH dosage of 2.5 g,oscillation speed of 800 r·min^(-1) and oscillation time of 4 min.Limits of detection of AM and MA were 0.14μg·L^(-1) and 1.15μg·L^(-1) respectively.Linear detection range was 5~75μg·L^(-1) with correlation coefficients of 0.995 and 0.994 respectively.The mean recoveries varied from 89.6%to 106.9%at spiked levels of 12.5 and 25μg·L^(-1),and the relative standard deviations(RSDs)ranged from 4.3%to 8.1%(n=6).This method adopts rapid pretreatment combined with GC-SAW,which has the advantages of simple operation,high detection sensitivity and fast speed.It is suitable for the preliminary screening of drug driving on-site detection in public security.

关 键 词：苯丙胺 甲基苯丙胺 快速前处理 声表面波快速气相色谱仪 唾液 毒驾 

分 类 号：O657.7[理学—分析化学]