A Glycosylated and Catechol-crosslinkedε-Polylysine Hydrogel:Simple Preparation and Excellent Wound Hemostasis and Healing Properties  

作　　者：Lin Teng Zheng-Wei Shao Yu-Shi He Jia-Yu Lu De-Rong Zou Chuan-Liang Feng Chang-Ming Dong 

机构地区：[1]School of Chemistry and Chemical Engineering,Frontiers Science Center for Transformative Molecules,Shanghai Key Laboratory of Electrical Insulation and Thermal Aging,Shanghai Jiao Tong University,Shanghai 200240,China [2]Department of Stomatology,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital,Shanghai Jiao Tong University,Shanghai 200233,China [3]School of Chemistry and Chemical Engineering,Shanghai Electrochemical Energy Devices Research Center,Shanghai Jiao Tong University,Shanghai 200240,China [4]School of Materials Science and Engineering,State Key Laboratory of Metal Matrix Composites,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Chinese Journal of Polymer Science》2022年第9期1110-1119,共10页高分子科学（英文版）

基　　金：financially supported by National Key Research and Development Project of China (No. 2021YFB4001101);the National Natural Science Foundation (NSFC) of China (No.22075176);Natural Science Foundation of Shanghai (No.22ZR1429200);NSFC projects (Nos. 51833006, 82071160,81870806 and 81974152)

摘　　要：Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs.Herein,we designed the glucose-and catechol-functionalized derivatives from commercialε-polylysine(EPL)and prepared the hydrogels by simple amidation and catechol-crosslinking reactions,which have larger swelling ratios of 220%–240%,suitable microporous size of about 6–8µm,and tissue adhesion strength of about 20–40 kPa.The hemolysis,cytotoxicity,and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73%±0.14%.The blood-clotting index,blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation,resulting in excellent hemostasis performance with a short hemostatic time of 38–46 s and less blood loss of 19%–34%in a liver hemorrhage model.A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0%±2.7%on postoperative day 7 and nearly full closure on day 14.Remarkably,the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55µg/mg comparable to that in normal skins on day 14,evidencing the wound healing was close to completion in the H-3 treatment.Consequently,this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL,holding translational potentials in wound hemostats and dressings.

关 键 词：Polylysine hydrogel Glycosylation Mussel-mimetic HEMOSTASIS Wound dressing 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]