电针对脓毒症大鼠肺炎症反应和高迁移率族蛋白B1的影响  

Effects of Electroacupuncture on Pneumonia Inflammation an High Mobility Group Protein B1 in Septic Rats

作　　者：王成 张晓燕 邵少举 赵波 万东君 张西京 WANG Cheng;ZHANG Xiao-yan;SHAO Shao-ju;ZHAO Bo;WAN Dong-jun;ZHANG Xi-jing(Department of neurology,the 940th Hospital of Joint Logistic Support Force of PLA,Lanzhou,Gansu,730050,China;Department of Critical Care Medicine,Xijing Hospital,Air Force Military Medical University,Xi'an,Shaanixi,710032,China)

机构地区：[1]联勤保障部队第940医院神经内科,甘肃兰州730050 [2]空军军医大学第一附属医院重症医学科,陕西西安710032

出　　处：《现代生物医学进展》2022年第13期2427-2430,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81272135)。

摘　　要：目的:研究电针足三里对脓毒症大鼠肺脏炎症反应和病理损伤的调节,为脓毒症的临床治疗提供新的理论依据。方法:成年雄性SD大鼠40只,按照随机数字表法分为假手术组(sham组)、脓毒症(盲肠结扎穿刺术(Cecal ligation and puncture,CLP)组)、脓毒症+假电针组(非经非穴组)和脓毒症+电针足三里组(足三里组),每组10只。除第一组外,其余大鼠均采用盲肠结扎穿孔术(CLP)制备脓毒症大鼠模型;造模前,脓毒症+假电针组选择非经非穴处电针刺激,脓毒症+电针足三里组给予足三里穴位电针处理,电针参数为疏密波,2 Hz,1 m A,持续30 min,连续5天。术后24 h,先取支气管肺泡灌洗液,再取右肺测湿干重比,取左肺下叶观察病理学改变,取左肺上叶检测炎症因子肿瘤坏死因子-α(Tumor necrosis factor,TNF-α)、白介素-1(interleukin-1,IL-1β)、白介素-6(interleukin-6,IL-6)和HMGB1。结果:与sham组比较,CLP组大鼠肺组织出现明显病理损伤(均P<0.05),炎症因子明显升高(均P<0.01),高迁移率族蛋白1(High mobility group 1 protein,HMGB1)明显升高(P<0.05);经过电针足三里处理,脓毒症大鼠肺组织病理损伤明显减轻(P<0.05),炎症因子明显降低(均P<0.01),HMGB1明显减少(P<0.05)。CLP组与非经非穴组大鼠生存率、肺组织病理损伤、炎症因子和HMGB1无明显差异,差异无统计学意义(均P>0.05)。结论:电针足三里可以减轻脓毒症大鼠肺的炎症反应和组织损伤,降低其肺脏HMGB1水平。Objective:To study the regulation of electroacupuncture Zusanli(ST36) on pulmonary inflammatory response and pathological injury in septic rats,and to provide a new theoretical basis for the clinical treatment of sepsis.Methods:A total of40 adult male SD rats were divided into sham eptic(SHAM group),sepsis group(CLP group),sepsis + sham electroacupuncture group(non-meridian and non-acupoint group) and sepsis + electroacupuncture Zusanli(ST36) group(Zusanli group) according to random number table,with 10 rats in each group.Except for the first group,all the other rats were treated with cecal ligation and perforation(CLP) to prepare the sepsis rat model.Before modeling,the sepsis + sham electroacupuncture group was given by electroacupuncture at non-meridian and non-acupoint points,and the sepsis + electroacupuncture Zusanli(ST36) group was given by electroacupuncture at Zusanli(ST36) points.The parameters of electroacupuncture were density wave,2 Hz,1 m A,lasting 30 min for 5 consecutive days.The bronchoalveolar lavage fluid was collected 24 h after surgery,the right lung was collected to measure the wet-dry weight ratio,the lower lobe of the left lung was collected to observe the pathological changes,and the upper lobe of the left lung was collected to detect the inflammatory factors TNF-α,IL-1β,IL-6 and HMGB1.Results:Compared with SHAM group,lung tissue in CLP group showed significant pathological damage(all P0.05).Conclusion:Electroacupuncture in Zusanli(ST36)can reduce the Pulmonary inflammation and tissue damage in septic rats,and reduce the level of lung HMGB1.

关 键 词：电针 足三里 脓毒症 肺 

分 类 号：R631.2[医药卫生—外科学]