含镁介孔生物活性玻璃/锌黄长石复合支架的成骨活性  

Osteogenic Activity of Mg-Containing Mesoporous Bioactive Glass/Hardystonite Composite Scaffold

作　　者：马振 孙伟 叶丹丹 赵晓兵 王国成[2] MA Zhen;SUN Wei;YE Dandan;ZHAO Xiaobing;WANG Guocheng(School of Materials Seience and Enginecring,Changzhou University,Changzhou 213164,Jiangsu,China;Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,Guangdong,China)

机构地区：[1]常州大学材料科学与工程学院,江苏常州213164 [2]中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《硅酸盐学报》2022年第7期1964-1971,共8页Journal of The Chinese Ceramic Society

基　　金：国家自然科学基金(51872318);江苏省研究生科研与实践创新计划(SJCX20-0974)。

摘　　要：骨修复支架的成骨活性和力学性能对其使用效果至关重要。以具有良好力学性能的锌黄长石(CaZnSiO,HT)为基体,以 Mg 掺杂的生物可降解介孔生物活性玻璃(MBG)为涂层,制备 Mg-M/HT 多孔复合支架。研究 Mg 掺杂对复合支架微观结构和成骨活性的影响。利用 X 射线衍射仪、透射电子显微镜和 Fourier 变换红外吸收光谱仪等手段对复合支架的物化性能进行表征。利用人骨髓间充质干细胞(h BMSCs)考察 Mg 掺杂对支架成骨活性的影响。结果表明:Mg 掺杂虽然一定程度上降低了 MBG 的介孔有序度,但 Mg-M/HT 复合支架仍具有均匀有序的孔道结构。与 MBG 改性的 HT 生物支架(M/HT)相比,Mg-M/HT 支架具有更高的细胞内黏着斑蛋白和整合素以及碱性磷酸酶、钙结节和骨钙素的表达量,表明 Mg 掺杂可促进 M/HT支架的成骨细胞活性,在骨组织修复领域具有较好的应用潜力。The osteogenic activity and mechanical properties of the bone repair scaffold are important for its clinical application.Mg-M/HT porous composite scaffold was prepared with hardystonite (CaZnSiO, HT) with good mechanical properties as a matrix and Mg-doped biodegradable mesoporous bioactive glass (MBG) as a coating. The effect of Mg doping on the microstructure and osteogenic activity of the M/HT composite scaffold was investigated. The microstructures of the composite scaffolds were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy(FTIR). The osteogenic activity of the scaffolds was investigated with human bone marrow mesenchymal stem cells (hBMSCs). The results show that Mg doping can reduce the mesoporous order of MBG to a certain extent, but Mg-M/HT composite scaffold still has a uniform and ordered pore structure. Compared with M/HT scaffold, Mg-M/HT scaffold has higher intracellular focal adhesion proteins and integrins, as well as alkaline phosphatase, calcium nodules and osteocalcin expression, indicating that Mg doping can promote the osteoblast activity of M/HT scaffold. Mg-M/HT scaffold has a potential application in the field of bone tissue repair.

关 键 词：骨修复 介孔生物活性玻璃 锌黄长石 镁 成骨活性 

分 类 号：R318[医药卫生—生物医学工程]