丁香酚诱导癌细胞PC-12凋亡的研究  

Study on the Apoptosis of PC-12 Cells Induced by Eugenol

作　　者：苏萌 吕洁 张兰 雷莉妍 SU Meng;LV Jie;ZHANG Lan;LEI Liyan(Shaanxi University of Chinese Medicine,Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research,Xianyang,Shaanxi 712083,China;The Affiliated Hospital to Shaanxi University of Chinese Medicine,Xianyang,Shaanxi 712000,China)

机构地区：[1]陕西中医药大学,陕西省中药资源产业化协同创新中心,陕西省中药基础与新药研究重点实验室,陕西咸阳712083 [2]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《今日药学》2022年第6期434-436,443,共4页Pharmacy Today

基　　金：陕西省教育厅重点科学研究计划项目(20JY011);陕西省重点研发计划项目(2022SF-090)。

摘　　要：目的研究丁香酚诱导大鼠嗜铬细胞瘤PC-12的凋亡作用机制。方法用不同浓度的丁香酚处理PC-12细胞,MTT法检测细胞相对存活率,荧光染色观察细胞凋亡情况;比色法检测Caspase-3酶活性;real-time PCR检测Bax和Bcl-2 mRNA水平的变化,Western blot检测蛋白水平的变化。结果丁香酚能显著降低PC-12细胞存活率,促进Bax mRNA及蛋白表达,抑制Bcl-2 mRNA及蛋白表达,进而提高Caspase-3酶活性。结论丁香酚可能通过上调Bax、下调Bcl-2表达,激活Caspase-3酶活性,诱导PC-12细胞凋亡。OBJECTIVE To investigate the mechanism of eugenol-induced apoptosis of pheochromocytoma PC-12 in rats.METHODS PC-12 cells were treated with different concentrations of eugenol.The relative survival rate of PC-12 cells was detected by MTT method,and the apoptosis was observed by fluorescence staining.Caspase-3 enzyme activity was detected by colorimetry.The mRNA levels of Bax and Bcl-2 were detected by real-time PCR,and the protein levels were detected by Western Blot.RESULTS Eugenol significantly decreased the survival rate of PC-12 cells,promoted the mRNA and protein expressions of Bax,inhibited the mRNA and protein expressions of Bcl-2,and increased the activity of Caspase-3.CONCLUSION Eugenol may induce PC-12 cell apoptosis by up-regulating Bax and down-regulating Bcl-2 expression,activating caspase-3 activity.

关 键 词：丁香酚 PC-12细胞 凋亡 

分 类 号：R96[医药卫生—药理学]