TGF-β1、α-SMA、Smad7在中药复方补肺理气汤治疗肺纤维化小鼠模型中的表达研究  

Expressions of TGF-β1,α-SMA and Smad7 in the mice model of pulmonary fibrosis treated with a Chinese herbal formula Bufei Liqi decoction

作　　者：郑旭旭 李丽燕[1] 方燕 李献超[1] ZHENG Xuxu;LI Liyan;FANG Yan(Department of Pathology,Wenzhou Hospital of Traditional Chinese Medicine,Wenzhou 325000,China)

机构地区：[1]温州市中医院病理科,浙江温州325000

出　　处：《全科医学临床与教育》2022年第8期697-701,712,F0002,共7页Clinical Education of General Practice

基　　金：温州市基础性科研项目(Y20210691)。

摘　　要：目的 研究中药复方补肺理气汤对肺纤维化小鼠模型转化生长因子-β1(TGF-β1)、平滑肌肌动蛋白(α-SMA)、母亲信号蛋白同源物7(Smad7)表达的影响。方法 选取60只SD小鼠,随机分为6组,每组10只,随机取1组为正常组,其余5组构建肺纤维化小鼠模型,分别为模型组,复方补肺理气汤低、中、高剂量组,醋酸泼尼松组。记录各组小鼠一般情况;末次灌胃后24 h取小鼠肺组织进行HE及Masson染色观察病理损伤;免疫组化法观察肺组织TGF-β1、α-SMA、Smad7蛋白表达;RT-qPCR法检测TGF-β1、α-SMA、Smad7 mRNA表达。结果 正常组小鼠精神状态良好,饮食正常,皮毛有光泽,体重稳定;构建模型后小鼠精神萎靡,进食减少,皮毛黯淡无光,体重降低;醋酸泼尼松及复方补肺理气汤用药后精神逐渐恢复,进食量逐渐增加,皮毛光泽度增加,体重也逐渐上升,与正常组小鼠基本相同。构建肺纤维化模型后,小鼠肺组织中可见大量炎症细胞浸润,给予复方补肺理气汤低、中、高剂量及醋酸泼尼松治疗后,肺组织形态改善,肺组织中炎症细胞浸润减少,纤维化程度逐渐缓解,与模型组比较差异显著,且复方补肺理气汤高剂量组及醋酸泼尼松组改变最为明显。与正常组比较,模型组TGF-β1、α-SMA蛋白表达明显增多,Smad7蛋白表达明显减少(LSD-t分别=16.88、13.89、-11.33,P均<0.05);与模型组比较,复方补肺理气汤低、中、高剂量组及醋酸泼尼松组TGF-β1、α-SMA蛋白表达减少,Smad7蛋白表达增加(LSD-t分别=5.11、9.29、15.15、15.02;3.74、7.93、12.91、13.13;4.42、8.92、14.78、12.67,P均<0.05)。模型组TGF-β1、α-SMA mRNA表达较正常组升高,Smad7 mRNA表达较正常组降低(LSD-t分别=22.52、20.00、-38.59,P均<0.05);复方补肺理气汤低、中、高剂量组及醋酸泼尼松组TGF-β1、α-SMA mRNA表达较模型组降低,Smad7 mRNA表达较模型组增加(LSD-t分别=8.42、13.79、21.50、21.28;8.14、12.77、18.79、Objective To investigate the effect of Chinese herbal formula Bufei Liqi decoction on TGF-β1,α-SMA,Smad7 expressions in the mice model of pulmonary fibrosis. Methods A total of 60 SD mice were randomly divided into 6 groups with 10 mice in each group.One group was randomly selected as normal group,and the other 5 groups constructed pulmonary fibrosis mouse models,including model group,compound Bufei Liqi decoction low-dose group,mediumdose group and high-dose group,and prednisone acetate group.The general condition of mice in each group was recorded.24h after the last intragastric administration,lung tissues of mice were collected for HE and Masson staining to observe pathological damage.The expressions of TGF-β1,α-SMA,and Smad7 protein in lung tissue were observed by immunohistochemistry.The mRNA expressions of TGF-β1,α-SMA and Smad7 were detected by RT-qPCR. Results Mice in normal group had good mental state,normal diet,shiny fur and stable body weight.After building the model,the mice were depressed,ate less,and lost weight. After receiving the treatment of prednisone acetate and compound Bufei Liqi decoction,the mental outlook gradually recovered,the food intake gradually increased,the fur gloss increased,the body weight also increased,and the mice were basically the same as the normal group.After the establishment of pulmonary fibrosis model,a large number of inflammatory cell infiltration could be seen in the lung tissues of mice.After treatment with compound Bufeiliqi decoction with low,medium and high doses and prednisone acetate,the morphology of lung tissues was improved,inflammatory cell infiltration in lung tissues was reduced,and the degree of fibrosis was gradually relieved,with significant differences when compared with the model group.The change of compound Bufei Liqi decoction with high-dose group and prednisone acetate group was the most obvious.Compared with normal group,TGF-β1,α-SMA protein expressions in model group increased significantly,Smad7 protein expression decreased significan

关 键 词：TGF-Β1 Α-SMA SMAD7 复方补肺理气汤 肺纤维化 

分 类 号：R285.5[医药卫生—中药学]