FSH诱导Hippo信号通路激素代谢相关基因表达的研究  

Expression of FSH-induced hormone metabolism-related genes of Hippo signaling pathway in human ovarian granulosa cells

作　　者：夏燕 练冰 孟夏 林丹换 李仕芬 王伟洪 覃春容[1] XIA Yan;LIAN Bing;MENG Xia;LIN Danhuan;LI Shifen;WANG Weihong;QIN Chunrong(Reproductive Medical Center,Shenzhen Maternal&Child Healthcare Hospital,Southern Medical University,Shenzhen 518000,Guangdong Province,China;Department of Gyneacology,Shenzhen Maternal&Child Healthcare Hospital,Southern Medical University,Shenzhen 518000,Guangdong Province,China;Department of the Traditional Chinese Medicine,Shenzhen University General Hospital,Shenzhen 518000,Guangdong Province,China)

机构地区：[1]南方医科大学附属深圳市妇幼保健院生殖医学科,广东深圳518000 [2]南方医科大学附属深圳市妇幼保健院妇科,广东深圳518000 [3]深圳大学总医院中医科,广东深圳518000

出　　处：《同济大学学报（医学版）》2022年第4期474-480,共7页Journal of Tongji University(Medical Science)

基　　金：广东省自然科学基金面上项目(2020A1515010096);深圳市卫计委学科能力提升项目(SZXJ2018037);深圳市医学重点学科项目(SZXK031);深圳市妇幼保健院院内博士科研基金(FYA2018015)。

摘　　要：目的探讨卵巢颗粒细胞中Hippo信号通路激素代谢相关基因的表达,为研究女性生殖内分泌异常疾病的发病机制提供理论基础。方法通过体外分离培养原代人卵巢颗粒细胞,检测人卵巢颗粒细中FSH诱导的Hippo信号通路Yes相关蛋白(Yes associated protein,YAP)、及其上下游相关基因,以及激素代谢相关基因的表达调控情况,明确FSH是否诱导Hippo信号通路激素代谢相关基因的调控表达。结果Hippo信号通路下游靶基因CTGF在不同FSH诱导剂量的诱导下表达均明显下调(P<0.001),而激素代谢相关基因Cyp19(P<0.001)、Ptgs2(P<0.001)、RUNX2(P<0.001)和Errb4(P<0.05)的表达明显上调,差异均有统计学意义。FSH诱导前后YAP基因及其下游TEAD家族相关转录因子表达差异无统计学意义。结论FSH可能诱导Hippo信号通路下游靶基因及相关激素代谢基因参与卵巢局部代谢调节。Objective To investigate the expression of hormone metabolism-related genes of Hippo signaling pathway in ovarian granulosa cells.Methods The expressions of Hippo signaling pathway Yes associated protein(YAP),its upstream and downstream related genes,and hormone metabolism-related genes before and after FSH induction were detected with RT-PCR in human ovarian granulosa cells.Results There was no significant difference in the expression of YAP gene and its downstream TEAD family-related transcription factors in the Hippo signaling pathway before and after FSH induction at the RNA level.CTGF as one of the downstream target genes of YAP was significantly down-regulated after FSH induction,and the Cyp19,Ptgs2 and RUNX2 expressions were all significantly up-regulated.Conclusion FSH may induce downstream target genes of Hippo signaling pathway and related hormone metabolism genes to participate in the regulation of the ovarian local metabolism.

关 键 词：Hippo信号通路 YAP基因 激素代谢 CTGF基因 TEAD基因家族 

分 类 号：R711.6[医药卫生—妇产科学]