佐剂性关节炎大鼠miR145-5p/Smads通路变化、巨噬细胞极化及其相关性分析  

Correlation analysis of changes in miR145-5p/Smads pathway and macrophage polarization in adjuvant arthritis rats

作　　者：范文杰 谌曦[2] 万磊[2] 范海霞[2] 刘天阳[2] 李明[2] 刘磊[2] 葛瑶[2] 王晴晴 费陈晨 周倩 FAN Wen-jie;SHEN Xi;WAN Lei;FAN Hai-xia;LIU Tian-yang;LI Ming;LIU Lei;GE Yao;WANG Qing-qing;FEI Chen-chen;ZHOU Qian(Graduate Department of Anhui University of Traditional Chinese Medicine Department of Rheumatology,Hefei 230038,China;The Hospital of Anhui University of Chinese Medicine,Hefei 230031,China)

机构地区：[1]安徽中医药大学研究生院,安徽合肥230038 [2]安徽中医药大学第一附属医院风湿科,安徽合肥230031

出　　处：《海南医学院学报》2022年第16期1222-1227,共6页Journal of Hainan Medical University

基　　金：安徽省高校自然科学研究项目(KJ2020A0396)。

摘　　要：目的:探究佐剂性关节炎大鼠miR145-5p/Smads通路变化与巨噬细胞极化之间的联系。方法:12只大鼠使用随机数字表法分成正常组和注射弗氏完全佐剂(0.1 mL/只)致炎的模型组,6只/组。大鼠关节炎形成后第12天,酶联免疫吸附试验检测滑膜组织中巨噬细胞极化标志物IL-8、CD206表达。免疫蛋白印记法检测滑膜组织中TGF-β1/Smads通路因子表达。RT-qPCR法检测滑膜组织中miR145-5p、Smads3、Smads7的表达。结果:与正常组相比,模型组大鼠IL-8、TGF-β1、Smad3的表达水平明显升高(P<0.05);CD206、Smad7、miR145-5p的表达水平显著降低(P<0.01)。相关性结果显示,IL-8与Smad3呈正相关(P<0.01),Smad7呈负相关(P<0.05);CD206与Smad3呈负相关(P<0.01),与Smad7呈正相关(P<0.05);miR145-5p与Smad3呈负相关(P<0.01),与Smad7呈正相关(P<0.01)。结论:miR145-5p可能通过抑制Smad3表达,进而抑制TGF-β1/Smads通路过激活,调节巨噬细胞极化,抑制佐剂性关节炎的发展。Objective:To explore the relationship between the changes of miR145-5p/Smads pathway and macrophage polarization in adjuvant arthritis rats. Methods:Twelve rats were divided into normal group and model group induced by freund’s complete adjuvant(0.1 mL/mouse)by random number table method,with 6 rats in each group. The expression of inflammatory polarization markers IL-8 and CD206 in synovial tissue was detected by enzyme-linked immunosorbent assay on the 12th day after the formation of arthritis in rats. Western blotting was used to detect the expression of TGF-β1/Smads pathway factors in synovial tissues. The expression of miR145-5P,Smads3 and Smads7 in synovial tissue was detected by RT-qPCR. Results:Compared with normal group,the expression levels of IL-8,TGF-β1 and Smad3 in model group were significantly increased(P<0.05);The expression levels of CD206,Smad7 and miR145-5P were significantly decreased(P<0.01). The correlation results showed that IL-8 was positively correlated with Smad3(P<0.01),IL-8 was negatively correlated with Smad7(P<0.05),CD206 was negatively correlated with Smad3(P<0.01)and positively correlated with Smad7(P<0.05). miR145-5p was negatively correlated with Smad3(P<0.01)and positively correlated with Smad7(P<0.01). Conclusion:miR145-5p may inhibit the overactivation of TGF-β1/Smads pathway,regulate macrophage polarization,and inhibit the development of adjuvant arthritis by inhibiting Smad3 expression.

关 键 词：佐剂关节炎 miR145-5p/Smads通路 巨噬细胞极化 

分 类 号：R593.22[医药卫生—内科学] R-332[医药卫生—临床医学]