花生不同组织内生真菌群落多样性及其生态功能结构分析  

Community Diversity and Ecological Functional Structural Analysis of Endophytic Fungi in Different Tissues of Arachis hypogaea Linn.

作　　者：李亮亮 王继雯[1] 刘德海[1] 甄静[1] 慕琦[1] 李磊 雷高 杨文玲[1] Li Liangliang;Wang Jiwen;Liu Dehai;Zhen Jing;Mu Qi;Li Lei;Lei Gao;Yang Wenling(Institute of Biology Co.,Ltd.,Henan Academy of Sciences/Key Laboratory of Microbial Engineering of Henan,Zhengzhou 450008,China)

机构地区：[1]河南省科学院生物研究所有限责任公司/河南省微生物工程重点实验室,河南郑州450008

出　　处：《山东农业科学》2022年第7期85-92,共8页Shandong Agricultural Sciences

基　　金：河南省科技攻关项目(222102110309);河南省科学院杰出青年人才培养项目(200405006);河南省科学院重大科研聚焦项目(210105002);河南省科学院助推科技成果转化项目(210205029,210205031);中原科技创新领军人才计划项目(214200510011)。

摘　　要：本试验通过高通量测序技术研究花生不同组织内生真菌群落多样性,一共获得763 200条有效序列,聚类后得到308个OTU,隶属于9个门,25个纲,51个目,107个科,155个属。根部内生真菌优势属为镰刀菌属(Fusarium)、曲霉属(Aspergillus)、丝孢酵母属(Trichosporon)、Apiotrichum和篮状菌属(Talaromyces),相对丰度分别为13.35%、10.74%、8.32%、6.29%和5.09%;茎部内生真菌优势属为Apiotrichum、丝孢酵母属(Trichosporon)、皮状丝孢酵母属(Cutaneotrichosporon)和曲霉属(Aspergillus),相对丰度分别为17.99%、13.81%、7.60%和5.92%;叶部内生真菌优势属为小光壳属(Leptosphaerulina)和暗球腔菌属(Phaeosphaeria),相对丰度分别为65.64%和27.18%;花部内生真菌优势属为小光壳属(Leptosphaerulina),相对丰度达到95.45%。经FUNGuild软件分析,花生内生真菌共包含地衣共生真菌、未定义腐生菌、动物病原菌、内生菌、木腐菌、植物病原菌、粪生菌、丛枝菌根真菌等17种生态功能群。Based on high-throughput sequencing technology, the community diversity of endophytic fungi in different tissues of Arachis hypogaea were analyzed.A total of 763 200 valid sequences and 308 OTUs were obtained, which belonged to 9 phyla, 25 classes, 51 orders, 107 families and 155 genera. The predominant genera in root were Fusarium, Aspergillus, Trichosporon, Apiotrichum and Talaromyces with relative abundance of 13.35%, 10.74%, 8.32%, 6.29% and 5.09%, respectively. The predominant genera in stem were Apiotrichum, Trichosporon, Cutaneotrichosporon and Aspergillus with relative abundance of 17.99%, 13.81%, 7.60% and 5.92%, respectively. The predominant genera in leaves were Leptosphaerulina and Phaeosphaeria with relative abundance of 65.64% and 27.18%, respectively. The predominant geneus in flower was Leptosphaerulina with relative abundance of 95.45%. The FUNGuild software analysis displayed that the endophytic fungi of Arachis hypogaea contained 17 functional groups including Lichenized, Undefined Saprotroph, Animal Pathogen, Endophyte, Wood Saprotroph, Plant Pathogen, Dung Saprotroph, Arbuscular Mycorrhizal, et al.

关 键 词：花生 高通量测序 内生真菌 生态功能群 

分 类 号：S182[农业科学—农业基础科学] S565.201