Na_(2)SeO_(3)-SA/CS微球/α-CPC基复合骨水泥的制备与性能  

Preparation and Properties of Na_(2)SeO_(3)-SA/CS Microspheres/α-CPC-based Composite Bone Cement

作　　者：李香香 冉青 毛敏 辛国鹏 刘信平 LI Xiangxiang;RAN Qing;MAO Min;XIN Guopeng;LIU Xinping(School of Chemical and Environmental Engineering,Hubei Minzu University,Enshi 445000,China)

机构地区：[1]湖北民族大学化学与环境工程学院,湖北恩施445000

出　　处：《合成化学》2022年第8期603-612,共10页Chinese Journal of Synthetic Chemistry

基　　金：国家自然科学基金重点项目(21461009)。

摘　　要：结合壳聚糖(CS)和海藻酸钠(SA)的生物相容性及元素硒的生理活性功能,研究功能性磷酸钙复合骨水泥的制备及性能。Na_(2)SeO_(3)为药物模型,乳化交联法制备Na_(2)SeO_(3)-SA/CS纳米微球,沉淀-煅烧-球磨法制备α-TCP,按10.0∶0.5∶0.5的物质的量比将α-TCP、DCPD和CaCO_(3)粉末混均磨匀制得α-CPC,以壳聚糖和柠檬酸复合物为固化液,将载硒微球与α-CPC调和制备Na_(2)SeO_(3)-SA/CS纳米微球/α-TCP基复合骨水泥。通过IR、DTG、DSC、SEM和XRD等手段表征其结构和性能,并采用原子荧光测定硒含量。探究球磨时间、载硒微球掺入量、固化液组分和固液比对复合骨水泥固化、强力、降解、抗溃散、缓释等性能的影响,并以MG63肉骨瘤细胞为模型进行体外抗肿瘤活性试验。结果表明:在球磨时间为4 h、复合固化液中固液比为1.0∶0.6(柠檬酸与壳聚糖的质量比为30∶1)、载硒微球的质量分数为5%的条件下制备的Na_(2)SeO_(3)-SA/CS/α-CPC复合骨水泥,相比于纯α-CPC,其降解速率和强度增大、抗溃散能力增强、固化时间略微缩短。SEM照片表明:缓释前圆滑的Na_(2)SeO_(3)-SA/CS微球被α-CPC均相包裹,缓释后形成了完善的三维蜂窝状多孔结构,利于细胞黏附和血管组织的长入。缓释前后的IR和XRD谱图表明:微球的掺入不影响CPC水化生成组分HA,只因降解率提升导致HA的量略微增加。复合骨水泥有效避免了Na_(2)SeO_(3)的突释现象,在40 d时硒累积释放率达32.34%,保证了活性硒的长时作用功效,肉骨瘤MG63细胞增殖抑制率在7 d时达到28.46%,故Na_(2)SeO_(3)-SA/CS/α-CPC复合骨水泥具有良好的细胞生物学效应和成骨活性。本文为植骨后所需的促修复、防止肿瘤细胞复发的功能性骨水泥材料的研究奠定了必要的实验基础。Combined with the biocompatibility of chitosan(CS)and sodium alginate(SA)and the physiological activity of elemental selenium,the preparation and properties of functional calcium phosphate composite bone cement were studied.NaSeO_(3) was used as a drug model.Na_(2)SeO_(3)-SA/CS nanospheres were prepared by emulsion cross-linking method,andα-TCP was prepared by precipitation-calcination-ball milling method.α-CPC was prepared by uniformly mixing and grindingα-TCP,DCPD and CaCO_(3) powders at the molar ratio of 10.0∶0.5∶0.5.Selenium-loaded microspheres were mixed withα-CPC with chitosan and citric acid complex as curing solution.Its structure and properties were characterized by IR,DTG,DSC,SEM and XRD,and the selenium content was determined by atomic fluorescence spectrometry.The effects of ball milling time,the content of selenium-loaded microspheres,the components of curing solution and the ratio of solid to liquid on the properties of composite bone cement such as curing,strength,degradation,anti-collapsibility,sustained release,etc.were investigated,and the antitumor activity of MG63 osteoma cells was tested in vitro.The results showed that the Na_(2)SeO_(3)-SA/CS/α-CPC composite bone cement prepared under the conditions of ball milling time of 4 h,solid-liquid ratio of composite curing solution of 1.0∶0.6(mass ratio of citric acid to chitosan is 30∶1)and mass ratio of selenium-loaded microspheres of 5%had higher degradation rate and strength,stronger anti-collapsibility and shorter curing time than pureα-CPC.SEM showed that the smooth Na_(2)SeO_(3)-SA/CS microspheres before sustained release were uniformly coated byα-CPC,and after sustained release,a perfect three-dimensional cellular porous structure was formed,which was beneficial to cell adhesion and vascular tissue growth.IR and XRD spectra before and after sustained release showed that the addition of microspheres did not affect the hydration of CPC to form HA,but the amount of HA increased slightly because of the increase of degradation rat

关 键 词：α-TCP Na_(2)SeO_(3)-SA/CS微球 α-CPC粉体 制备与性能 磷酸钙复合骨水泥 

分 类 号：O611[理学—无机化学]