雷公藤甲素对急性髓性白血病细胞增殖与凋亡的影响  

Effect of Triptolide on Proliferation and Apoptosis in Acute Myeloid Leukemia Cells

作　　者：胡仁智[1] 夏敏[1] 赵世巧[1] Hu Renzhi;Xia Min;Zhao Shiqiao(Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China)

机构地区：[1]重庆市中医院,重庆400021

出　　处：《中国中医急症》2022年第8期1188-1190,1205,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：成都中医药大学2020年度“杏林学者”医院专项(YYZX2020046)。

摘　　要：目的探讨雷公藤甲素引起急性髓性白血病细胞凋亡的相关分子机制。方法使用不同浓度雷公藤甲素处理HL-60,U937细胞24 h后用MTT法检测细胞存活率,流式细胞仪检测凋亡,Western blotting检测P62、LC3B、Caspase-3、PARP-1及细胞色素C,联用自噬抑制剂3-MA预处理U937细胞后流式细胞仪检测凋亡,自噬以及凋亡相关蛋白用蛋白印迹法检测。结果雷公藤甲素对HL-60、U937细胞的IC50值分别为(33.86±2.84)、(36.67±3.15)nmol/L,雷公藤甲素可明显诱导HL-60、U937细胞凋亡,差异有统计学意义(P<0.01)。雷公藤甲素可降低自噬降解底物蛋白P62表达,自噬标志蛋白LC3B-II表达上调,说明雷公藤甲素可诱导自噬。自噬抑制剂3-甲基腺嘌呤预处理细胞,阻断凋亡信号通路的激活,雷公藤甲素诱导的白血病细胞凋亡明显减少。结论雷公藤甲素可促进自噬体降解进而激活凋亡信号通路,诱导急性髓性白血病细胞凋亡。Objective:To explore the effect of triptolide on proliferation and apoptosis of acute myeloid leukemia(AML)cells.Methods:AML cells were treated with different concentrations of TPL for 24 h and MTT assay was used to measure the viability of cells.Cell apoptosis were assessed by flow cytometry with Annexin V-/PI staining.Changes in P62,LC3B,Caspase-3,PARP-1 and Cytochrome C levels were measured by Western blotting.U937 cells were treated with autophagy inhibitor 3-MA and then flow cytometry was used to detect the apoptosis.The re⁃lated proteins about apoptosis and autophagy were assessed by WB analysis.Results:The half survival rate of U937 and HL-60 cells treated with triptolide was(36.67±3.15)nmol/L vs(33.86±2.84)nmol/L(P<0.01).Trip⁃tolide resulted in marked decreases in cell viability and increases in apoptosis(P<0.01)in U937 and HL-60 cells in dose dependent manners.Western blot showed that treatment with triptolide increased the levels of LC3BII and decreased the levels of P62 in whole-cell.Inhibition of autophagy by 3-MA significantly attenuated apopto⁃sis mediated by triptolide treatment(P<0.01).Conclusion:Triptolide promotes autophagosome degradation and activates apoptosis signaling pathway to induce apoptosis in AML cells.

关 键 词：急性髓性白血病 雷公藤甲素 自噬 凋亡 

分 类 号：R285.5[医药卫生—中药学]