9种引进栎属植物遗传多样性及亲缘关系的ISSR分析  

Genetic Diversities and Relationships of 9 Introduced Species/Cultivars of Quercus by ISSR Molecular Markers

作　　者：余敏芬[1,2,3] 梅家琪 何立平 李东宾 徐栋斌 杨影 袁虎威 闫道良 郑炳松[1,2] YU Min-fen;MEI Jia-qi;HE Li-ping;LI Dong-bin;XU Dong-bin;YANG Ying;YUAN Hu-wei;YAN Dao-liang;ZHENG Bing-song(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions,Hangzhou 311300,China;Ningbo Forest Farm of Zhejiang,Ningbo 315440,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300 [2]浙江农林大学浙江省森林芳香植物康养功能研究重点实验室,浙江杭州311300 [3]宁波市林场,浙江宁波315440

出　　处：《浙江林业科技》2022年第5期9-15,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：宁波市科学技术局市级科技计划项目(CX-D01-科技富民项目2017C10014)。

摘　　要：为有效利用栎属Quercus植物,以‘针栎’Q.palustris‘Pin Oak’、‘猩红橡木’Q.coocinea‘Scarlet Oak’、‘太平洋光辉’Q.palustris‘Pacific Brilliance’、‘沼泽白橡木’Q.icolor‘Swamp White Oak’、‘北方红橡’Q.rubra‘Red Oak’、‘绿柱’Q.palustris‘Green pillar’、柳叶栎Q.phellos和2种娜塔栎Q.nuttallii种质(红叶娜塔栎和绿叶娜塔栎)9种国外引进的栎属植物为研究对象,利用ISSR分子标记对其遗传多样性和亲缘关系进行分析和UPGMA聚类。结果表明,在所选26条引物中筛选出23条多态性ISSR引物;利用多态性ISSR引物对9种栎属植物进行PCR扩增,共获得298条多态性条带,其多态性条带比例达93.42%,表明这9种栎属植物的遗传多样性较为丰富;9种栎属植物之间的遗传相似系数为0.4859~0.7649。UPGMA聚类结果表明,9种栎属植物之间具有不同程度的相对亲缘关系,在遗传相似系数0.650处,9种栎属植物可聚为3类,其中3个沼生栎品种(‘绿柱’‘太平洋光辉’和‘针栎’)和2种娜塔栎种质(红叶娜塔栎和绿叶娜塔栎)被归为第一类,柳叶栎、‘猩红橡木’以及‘北方红橡’被归为第二类,而‘沼泽白橡木’单独归为第三类,在第一类中,‘绿柱’和‘太平洋光辉’的亲缘关系最近,与其属于同一个种的分类特征一致,‘沼泽白橡木’与其他8个种(品种)间的相对亲缘关系最远。以上研究结果可为栎属植物的培育和开发利用提供参考。In October 2017,Quercus palustris‘Pin Oak’,Q.coocinea‘Scarlet Oak’,Q.palustris‘Pacific Brilliance’,Q.icolor‘Swamp White Oak’,Q.rubra‘Red Oak’,Q.palustris‘Green Pillar’were introduced from Haining,Zhejiang province,and in November 2017,5-year seedlings of Q.phellos and two Q.nuttallii germplasms(red leaves and green leaves)were introduced from Changsha,Hunan province,Ningbo,Zhejiang province.In May 2018,leaves of above introduced plants were collected for genomic DNA extract.Genetic diversities,genetic relationships were analyzed by UPGMA dendrogram and ISSR molecular markers.The results showed that 23 polymorphic primers were selected from 26 ones for ISSR,and 298 polymorphic bands accounting for 93.42%of the total bands were obtained by PCR amplification.The genetic similarity coefficients of species and cultivars of Quercus varied from 0.4859 to 0.7649.UPGMA dendrogram showed that there were different genetic relations among tested species and cultivars.They could be divided into 3 groups at the genetic similarity coefficient of 0.650,among which Q.palustris‘Green pillar’,Q.palustris‘Pacific Brilliance’and Q.palustris‘Pin Oak’and two Q.nuttallii germplasm were in Group I,Q.phellos,Q.coocinea‘Scarlet Oak’and Q.rubra‘Red Oak’were in group II,while Q.icolor‘Swamp White Oak’in group III.The relationship between Q.palustris‘Green pillar’and Q.palustris‘Pacific Brilliance’was the closest,because of the same species,while the relationships between Q.icolor‘Swamp White Oak’and the other 8 species/culitvars were the farthest.

关 键 词：栎属 ISSR分子标记 遗传多样性 亲缘关系分析 

分 类 号：S792.18[农业科学—林木遗传育种]