A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication  

作　　者：Zeyu Zhang Chenming Wu Chengkai Dai Qingqing Shi Guoxin Fang Dongfang Xie Xiangjie Zhao Yong-Jin Liu Charlie CLWang Xiu-Jie Wang 

机构地区：[1]Institute of Genetics and Developmental Biology,Innovation Academy of Seed Design,Chinese Academy of Sciences,Beijing,100101,China [2]Beijing National Research Center for Information Science and Technology,Department of Computer Science and Technology,Tsinghua University,Beijing,100084,China [3]Department of Mechanical,Aerospace and Civil Engineering,The University of Manchester,Manchester,M204BX,UK [4]Faculty of Industrial Design Engineering,Delft University of Technology,Delft,2628,the Netherlands [5]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Bioactive Materials》2022年第12期138-150,共13页生物活性材料（英文）

基　　金：CAS Strategic Priority Research Program grants(XDA16020801 to X.-J.W.);the National Natural Science Foundation of China(81790622 to X.-J.W.and 61725204 to Y.-J.L.)。

摘　　要：Despite the recent advances in artificial tissue and organ engineering,how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative medicine.Three-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues,yet it still faces difficulties to generate vasculatures and preserve cell functions in complex organ production.Here,we overcome the limitations of conventional bioprinting systems by converting a six degree-of-freedom robotic arm into a bioprinter,therefore enables cell printing on 3D complex-shaped vascular scaffolds from all directions.We also developed an oil bath-based cell printing method to better preserve cell natural functions after printing.Together with a self-designed bioreactor and a repeated print-and-culture strategy,our bioprinting system is capable to generate vascularized,contractible,and long-term survived cardiac tissues.Such bioprinting strategy mimics the in vivo organ development process and presents a promising solution for in vitro fabrication of complex organs.

关 键 词：Six degree-of-freedom robot 3D bioprinting Artificial organ engineering Print-and-culture Cardiac tissue fabrication 

分 类 号：R318[医药卫生—生物医学工程] TB3[医药卫生—基础医学]