In vitro degradation,photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca-P coating on magnesium alloy AZ31  

作　　者：Zhen-Yu Zhang Yan-Lin An Xiao-Shi Wang Lan-Yue Cui Shuo-Qi Li Cheng-Bao Liu Yu-Hong Zou Fen Zhang Rong-Chang Zeng 

机构地区：[1]College of Materials Science and Engineering,Shandong University of Science and Technology,Qingdao,266590,China [2]College of Chemical and Biological Engineering,Shandong University of Science and Technology,Qingdao,266590,China [3]School of Materials Science and Engineering,Zhengzhou University,Zhengzhou,450002,China

出　　处：《Bioactive Materials》2022年第12期284-299,共16页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(No.52071191,52101288);Shandong Provincial Natural Science Foundation,China(ZR2020QE009).

摘　　要：Surgical failures,caused by postoperative infections of bone implants,are commonly met,which cannot be treated precisely with intravenous antibiotics.Photothermal therapy(PTT)and photodynamic therapy(PDT)have attracted widespread attention due to their non-invasive antibacterial effects on tissues and no bacterial resistance,which may be an excellent approach to solve infections related to bone implants for biodegradable magnesium alloys.Herein,a sodium copper chlorophyllin(SCC)with a porphyrin ring induced Ca-P coating was prepared on AZ31 magnesium alloy via layer-by-layer(LbL)assembly.The morphology and composition of the samples were characterized through field emission scanning electron microscope(FE-SEM)with affiliated energy dispersive spectrometer(EDS),X-ray diffractometer(XRD),and Fourier infrared spectrometer(FTIR)and X-ray photoelectron spectrometer(XPS)as well.Potentiodynamic polarization,electrochemical impedance spectroscopy(EIS)and hydrogen evolution experiments were employed to evaluate the corrosion behavior of the samples.Atomic absorption spectrophotometer was used to measure Cu elemental content of different immersion periods.Cytocompatibility and antibacterial performance of the coatings were probed using in vitro cytotoxicity tests(MTT assay),live/dead cell staining and plate counting method.The results showed that the obtained(Ca-P/SCC)10 coating exhibited good corrosion resistance,antimicrobial activity(especially under 808 nm irradiation)and biocompatibility.The antibacterial rates for E.coli and S.aureus were 99.9%and 99.8%,respectively;and the photothermal conversion efficiency was as high as 42.1%.Triple antibacterial mechanisms including photodynamic,photothermal reactions and copper-ions release were proposed.This coating exhibited a promising application for biodegradable magnesium alloys.

关 键 词：Magnesium alloy COATING Degradable biomaterial BIOCOMPATIBILITY Antibacterial activity 

分 类 号：R318[医药卫生—生物医学工程] TB3[医药卫生—基础医学]