基于解表扩络法探讨小青龙汤对肺动脉高压模型大鼠血管内皮素的影响  

Study on the effect of Xiaoqinglong decoction on vascular endothelin inpulmonary hypertension rat based on Jiebiaokuoluo method

作　　者：徐小璐 张金波 王蕊[3] 张雯鑫 商立珍 张正辉 Xu Xiaolu;Zhang Jinbo;Wang Rui;Zhang Wenxin;Shang Lizhen;Zhang Zhenghui(Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Preventive Treatment of Disease,Yantai Hospital of Traditional Chinese Medicine,Yantai 264013,Shandong,China;Department of Pharmacy,the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Yantai Vocational College of Culture and Tourism,Yantai 264600,Shandong,China;Department of Pulmonology,Yantai Hospital of Traditional Chinese Medicine,Yantai 264013,Shandong,China;Department of Geratology,Yantai Hospital of Traditional Chinese Medicine,Yantai 264013,Shandong,China)

机构地区：[1]山东中医药大学,济南250355 [2]烟台市中医医院治未病中心,山东烟台264013 [3]山东中医药大学第二附属医院药学部,济南250355 [4]烟台文化旅游职业学院,山东烟台264600 [5]烟台市中医医院肺病科,山东烟台264013 [6]烟台市中医医院老年病科,山东烟台264013

出　　处：《中华危重病急救医学》2022年第6期597-601,共5页Chinese Critical Care Medicine

基　　金：山东省中医药科技发展计划项目(2019-0670);齐鲁医派中医学术流派传承项目(2021-45)。

摘　　要：目的基于解表扩络法探讨小青龙汤对肺动脉高压(PAH)模型大鼠血管内皮素(ET)的影响。方法将60只雄性SD大鼠随机分为空白对照组、PAH模型组、阳性药物组及小青龙汤高、中、低剂量组,每组10只。空白对照组不进行任何处理。其余各组大鼠均饲养于低压氧箱内,分别于第1天和第14天注射脂多糖(LPS),每次200μL;第2~30天(第14天除外)每日烟熏2次(每次间隔4 h),期间每日置于低温环境下冷冻1 h,待大鼠恢复正常后放入冷水中游泳(持续2周),同时给予寒凉饮食。制模后,空白对照组和PAH模型组给予等体积生理盐水;阳性药物组给予100 mg/kg波生坦片;小青龙汤高、中、低剂量组分别给予小青龙汤15、10、5 g/kg;各组均每日1次,持续30 d。之后采用右心导管检查测定平均肺动脉压(mPAP)和右心室收缩压(RVSP),并评估右心室肥厚指数(RVHI);采用放射免疫法测定ET-1和一氧化氮(NO)水平。结果与空白对照组相比,PAH模型组大鼠mPAP、RVSP、RVHI和ET-1水平均明显升高〔mPAP(mmHg,1 mmHg≈0.133 kPa):33.20±1.04比13.20±1.03,RVSP(mmHg):62.40±1.54比24.20±1.02,RVHI:42.90±2.51比25.40±2.01,ET-1(ng/L):100.80±20.34比81.50±13.84,均P<0.05〕,NO水平明显降低(mmol/L:23.20±1.81比31.70±1.49,P<0.05)。与PAH模型组相比,阳性药物组及小青龙汤高、中、低剂量组大鼠mPAP、RVSP、RVHI和ET-1水平均明显降低〔mPAP(mmHg):25.50±0.84、26.90±0.74、27.10±1.19、29.10±0.75比33.20±1.04,RVSP(mmHg):54.40±5.14、50.10±1.67、53.10±1.05、56.60±1.07比62.40±1.54,RVHI:41.10±1.19、31.20±1.67、31.30±1.89、40.30±1.88比42.90±2.51,ET-1(ng/L):70.70±7.89、69.90±2.92、71.70±4.32、73.90±5.19比100.80±20.34,均P<0.05〕,NO水平明显升高(mmol/L:32.50±2.06、34.70±1.16、32.70±1.33、30.10±1.19比23.20±1.81,均P<0.05),且随小青龙汤剂量增加变化更明显,以大剂量组效果更好。结论小青龙汤可通过调控ET-1和NO水平减轻大鼠PAH,且呈剂量�Objective To discuss the effect of Xiaoqinglong decoction on vascular endothelin(ET)in rats pulmonary hypertension(PAH)model based on the Jiebiaokuoluo method.Methods Sixty male SD rats were randomly divided into six groups:control group,PAH model group,positive drug group,and Xiaoqinglong decoction in the high-dose,medium-dose and low-dose groups,10 rats in each group.The control group did not receive any treatment.The remaining groups were kept in low-pressure oxygen tanks,injections of lipopolysaccharide(LPS)at 200μL on day 1 and day 14,respectively.The rats were smoked twice a day from day 2 to day 30(except day 14)at 4 hours intervals.The rats were placed in a low-temperature environment for 1 hour per day,and were put into cold water to swim(for 2 weeks),while the rats were given a cold diet.After modeling,the control group and the PAH model group were given equal volumes of normal saline;the positive drug group was given bosentan(100 mg/kg);Xiaoqinglong decoction 15,10 and 5 g/kg was given,respectively,in the high,medium and low dose groups;once daily for 30 days.The mean pulmonary artery pressure(mPAP)and right ventricle systolic pressure(RVSP)were then measured with right heart catheterization and the right ventricle hypertrophy index(RVHI)was assessed;ET-1 and nitric oxide(NO)levels were determined by radioimmunoassay.Results Compared with the control group,the levels of mPAP,RVSP,RVHI and ET-1 were significantly increased in the PAH model group[mPAP(mmHg,1 mmHg≈0.133 kPa):33.20±1.04 vs.13.20±1.03,RVSP(mmHg):62.40±1.54 vs.24.20±1.02,RVHI:42.90±2.51 vs.25.40±2.01,ET-1(ng/L):100.80±20.34 vs.81.50±13.84,all P<0.05],while NO levels were significantly decreased(mmol/L:23.20±1.81 vs.31.70±1.49,P<0.05).Compared with the PAH model group,the levels of mPAP,RVSP,RVHI and ET-1 in the positive drug group and high,medium,low dose groups of Xiaoqinglong decoction were significantly decreased[mPAP(mmHg):25.50±0.84,26.90±0.74,27.10±1.19,29.10±0.75 vs.33.20±1.04,RVSP(mmHg):54.40±5.14,50.10±1.67,53.10

关 键 词：解表扩络 小青龙汤 血管内皮素 微观辨证 靶点治疗 

分 类 号：R285.5[医药卫生—中药学]