全自动凝血分析仪检测2种诱导剂诱导的血小板聚集试验参考区间的建立  

Establishment of reference interval for platelet aggregation test by automated coagulation analyzers with two different agonists

作　　者：寿玮龄[1] 陈倩[1] 徐雯[2] 马超超 吴卫[1] SHOU Weiling;CHEN Qian;XU Wen;MA Chaochao;WU Wei(Department of Laboratory Medicine,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730;Department of Laboratory Medicine,The Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,Henan,China)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]新乡医学院第三附属医院检验科,河南新乡453000

出　　处：《临床检验杂志》2022年第7期521-524,共4页Chinese Journal of Clinical Laboratory Science

摘　　要：目的基于全自动凝血分析仪血小板聚集试验通道,建立表观健康人群血小板最大聚集率(MPAR)的参考区间。方法募集表观健康人134例,采用Sysmex CN3000和Sysmex CS5100全自动凝血分析仪进行不同终浓度二磷酸腺苷(ADP)和花生四烯酸(AA)诱导的血小板聚集试验检测。根据美国临床和实验室标准协会(CLSI)EP28-A3c文件,按照非参数法计算MPAR的参考区间。结果不同终浓度ADP和AA诱导的MPAR重复性为2.03%~4.92%。不同终浓度ADP和AA诱导的MPAR在不同性别和年龄组之间差异均无统计学意义(P均>0.05)。2个检测系统各自ADP终浓度2和5μmol/L之间、CS系列AA终浓度0.5和1.0 mmol/L之间MPAR差异均有统计学意义(P均<0.05)。除AA终浓度1.0 mmol/L(Z=-2.319,P=0.020),余不同终浓度诱导的MPAR在2个系统间差异均无统计学意义(P均>0.05)。ADP终浓度2、5和10μmol/L诱导的MPAR生物参考区间在CN3000分别为45.4%~95.7%、72.0%~94.3%和76.3%~95.1%,在CS5100分别为45.3%~93.7%、73.1%~99.1%和75.9%~97.6%;AA终浓度0.5和1.0 mmol/L诱导的MPAR参考区间在CN3000分别为72.1%~95.7%和81.0%~95.9%,在CS5100分别为77.1%~96.9%和81.1%~99.5%。结论本研究初步建立了CN3000和CS5100不同终浓度ADP和AA诱导的血小板聚集试验MPAR参考区间。Objective To establish a reference range for maximum platelet aggregation rate(MPAR)for the apparent healthy population based on the platelet aggregation test channel in fully-automated coagulation analyzers.Methods A total of 134 healthy adults were enrolled.Platelet aggregation tests induced by different concentrations of adenosine diphosphate(ADP)and arachidonic acid(AA)were performed on the fully automated coagulation analyzers,CN3000 and CS5100,respectively.According to the Clinical and Laboratory Standards Institute(CLSI)EP28-A3 c documents,the reference range of maximum platelet aggregation rates(MPAR)was established by the non-parametric method.Results The repeatabilities of ADP and AA induced MPAR with different final concentrations were 2.03% to 4.92%.No significant difference of MPAR in different genders and ages was found(all P>0.05).There were significant differences in the results of MPAR obtained using ADP at 2 and 5μmol/L on the both systems,and using AA at 0.5 and 1.0 mmol/L on CS5100 system(all P<0.05).There was no significant difference for MPAR using the same concentration in the both test systems(all P>0.05)except using AA at 1.0 mmol/L(Z=-2.319,P=0.020).The reference ranges of ADP induced MPAR with final concentration 2,5 and 10μmol/L were 45.4% to 95.7%,72.0% to 94.3% and 76.3% to 95.1% on CN3000,and 45.3% to 93.7%,73.1% to 99.1% and 75.9% to 97.6% on CS5100,respectively.The reference ranges of AA induced MPAR with final concentration 0.5 and 1.0 mmol/L were 72.1% to 95.7% and 81.0% to 95.9% on CN3000,77.1% to 96.9% and 81.1% to 99.5% on CS5100,respectively.Conclusion The reference ranges of MPAR in platelet aggregation tests induced by different final concentrations of ADP and AA on automated coagulation analyzers CN3000 and CS5100 were preliminary established in this study.

关 键 词：血小板聚集试验 光学比浊法 参考区间 全自动凝血分析仪 

分 类 号：R446[医药卫生—诊断学]