菟丝子黄酮对大鼠种植窗期子宫内膜容受性的影响  

Effect of Flavonoids in Cuscuta Chinensi Lam.on Endometrial Receptivity during the Implantation Window in Rats

作　　者：李莹[1] 陈建玲[1] 吴佩蔚[1] 黄亚哲[1] 张帅[1] 张丹[2] LI Ying;CHEN Jian-Ling;WU Pei-Wei;HUANG Ya-Zhe;ZHANG Shuai;ZHANG Dan(Dept.of Reproductive Medicine,Nanyang Central Hospital,Nanyang 473400 Henan,China;Dept.of Reproductive Medicine,The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450000 Henan,China)

机构地区：[1]南阳市中心医院生殖医学科,河南南阳473400 [2]郑州大学第二附属医院生殖医学部,河南郑州450000

出　　处：《广州中医药大学学报》2022年第8期1886-1892,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河南省医学科技攻关计划联合共建项目(编号:LHGJ20190319)。

摘　　要：【目的】观察菟丝子黄酮对大鼠种植窗期子宫内膜容受性的影响。【方法】将40只胚胎种植窗期大鼠皮下注射米非司酮溶液建立胚胎着床障碍模型后,随机分为模型组、Stattic[信号转导和转录激活因子3(STAT3)抑制剂]+中药组、中药组、阳性对照组,每组10只,另设正常组10只。在妊娠第1~5天:Stattic+中药组先后给予Stattic混悬液3.75 mg/kg、菟丝子黄酮混悬液530.1 mg/kg灌胃;中药组给予530.1 mg/kg菟丝子黄酮混悬液灌胃;阳性对照组给予阿司匹林混悬液4.8 mg/kg灌胃;正常组和模型组给予等体积生理盐水(4 mL/kg)灌胃。每日1次。给药结束后,观察大鼠种植窗期一般情况,酶联免疫吸附分析(ELISA)检测血清雌二醇(E2)、孕酮(P)水平,观察着床胚泡情况,苏木素-伊红(HE)染色法观察大鼠子宫内膜组织病理形态,免疫组织化学染色法检测子宫内膜微血管密度(MVD),蛋白免疫印迹(Western Blot)法检测大鼠子宫内膜STAT3信号通路相关蛋白表达。【结果】模型组、Stattic+中药组、中药组、阳性对照组、正常组,大鼠子宫内膜胚泡着床数目依次增多(P<0.05)。模型组子宫空腔扩张,间质细胞致密,无蜕膜化表现,炎性细胞浸润严重;与模型组比较,Stattic+中药组、中药组及阳性对照组大鼠子宫内膜间质相对疏松,有蜕膜化表现,血管相对丰富,炎性细胞浸润明显减少。模型组、Stattic+中药组、中药组、阳性对照组、正常组,大鼠子宫内膜MVD及血清E2、P水平依次升高(P<0.05)。模型组、Stattic+中药组、中药组、正常组,大鼠宫内膜白血病抑制因子(LIF)、磷酸化STAT3(p-STAT3)蛋白相对表达量依次升高(P<0.05)。【结论】菟丝子黄酮可能通过激活STAT3信号通路改善大鼠种植窗期子宫内膜容受性。Objective To observe the effect of flavonoids in Cuscuta Chinensi Lam.on endometrial receptivity during the implantation window in rats.Methods Forty rats were injected subcutaneously with Mifepristone solution at the embryo implantation window to establish a rat model of embryo implantation disorder,and then randomly divided into the model group,the Stattic+Chinese herbal medicine group,the Chinese herbal medicine group,and the positive control group,10 rats in each group,and 10 rats set as another normal group.On day 1-5 of gestation,the Stattic+Chinese herbal medicine group was given Stattic suspension 3.75 mg/kg and Cuscuta flavonoids suspension 530.1 mg/kg by gavage;the Chinese herbal medicine group was given 530.1 mg/kg of Cuscuta flavonoids suspension by gavage;the positive control group was given Aspirin suspension 4.8 mg/kg by gavage;the normal group and the model group were given equal volume of physiological saline(4 mL/kg)by gavage.The rats were administered once daily.After administration,the general conditions of the implantation window of the rats were observed,the levels of serum estradiol(E2)and progesterone(P)were measured by enzyme-linked immunosorbent assay(ELISA),the condition of the implanted blastocysts was observed,the histopathological morphology of the rat endometrium was examined by hematoxylin-eosin(HE)staining,the microvascular density(MVD)of the endometrium was measured by immunohistochemical staining.Western Blot method was used to detect the expression of proteins related to signal transduction and activator of transcription 3(STAT3)signaling pathway in rat endometrium.Results In the model group,Stattic+Chinese herbal medicine group,Chinese herbal medicine group,positive control group and normal group,the number of endometrial blastocysts in the rats was increased in order(P<0.05).In the model group,the uterine cavity was dilated,the mesenchymal cells were dense,without metaplasia,and the inflammatory cell infiltration was severe;compared with the model group,the mesenchymal cells

关 键 词：菟丝子黄酮 子宫内膜容受性 种植窗期 信号转导和转录激活因子3信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]