基于Bcl-2/Bax凋亡探讨仙子益真胶囊对大鼠卵巢储备功能减退的作用机制  

Study on the Mechanism of Action of Xianzi Yizhen Capsules on Diminished Ovarian Reserve Function in Rats Based on Bcl-2/Bax Apoptosis

作　　者：温丹婷 彭潇[2] 郑玮琳 徐珉 WEN Dan-Ting;PENG Xiao;ZHENG Wei-Lin;XU Min(The Second Clinical Medical School of Guangzhou University of Chinese Medicine,Guangzhou 510045 Guangdong,China;Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120 Guangdong,China;Guangdong Second Provincial General Hospital,Guangzhou 510310 Guangdong,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510045 [2]广东省中医院,广东广州510120 [3]广东省第二人民医院,广东广州510310

出　　处：《广州中医药大学学报》2022年第7期1615-1621,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家中医药管理局项目:李丽芸全国名老中医药专家传承工作室(编号:1199ws02);广东省中医药局科研课题(编号:20201160)。

摘　　要：【目的】基于Bcl-2/Bax凋亡途径,探讨仙子益真胶囊(熟地黄、菟丝子等)对卵巢储备功能减退大鼠的作用机制。【方法】选用动情周期规律的60只SPF级雌性大鼠,随机分为正常组,模型组,中药低、中、高剂量组,西药组,每组10只。除正常组,其余组别大鼠构建卵巢储备功能减退模型。造模14 d后给药,中药低、中、高剂量组分别给予0.625、1.25、2.5 g·kg^(-1)·d^(-1)仙子益真胶囊灌胃,西药组给予补佳乐、地屈孕酮灌胃序贯治疗。干预28 d后,选择动情间期取材,实时定量聚合酶链反应(RT-qPCR)法检测卵巢组织凋亡因子Bcl-2、Bax、Caspase-3、p53 mRNA表达水平,蛋白免疫印迹(Western Blot)法检测卵巢组织核转录因子kappaB(NF-κB)p65、子宫内膜ERα、PR的蛋白表达水平。【结果】与正常组比较,模型组卵巢组织Bcl-2 mRNA表达水平降低,Bax、Caspase-3、p53 mRNA表达水平升高,Bcl-2/Bax比率降低,卵巢组织NF-κB p65,子宫内膜ERα、PR蛋白表达水平降低(均P<0.05);与模型组比较,中药组卵巢组织Bcl-2 mRNA表达水平升高,Bax、Caspase-3、p53 mRNA表达水平降低,Bcl-2/Bax比率升高,卵巢组织NF-κB p65与子宫内膜ERα、PR蛋白表达水平升高(均P<0.05)。【结论】仙子益真胶囊可有效改善雷公藤多苷导致的大鼠卵巢储备功能减退,提高卵巢储备功能,改善子宫内膜容受性。Objective To explore the mechanism of Xianzi Yizhen Capsules on rats with diminished ovarian reserve(DOR)by focusing on the Bcl-2/Bax apoptotic pathway.Methods Sixty female rats of SPF class with regular motility cycle were selected and randomly divided into normal group,model group,Chinese herbal medicine low-,medium-and high-dose groups and western medicine group,with 10 rats in each group.Except for the normal group,the rats in the rest of the groups were constructed as a DOR model.After 14 days of modeling,the rats in the Chinese herbal medicine low-,medium-and high-dose groups were given 0.625,1.25 and 2.5 g·kg^(-1)·d^(-1)of Xianzi Yizhen Capsules by gavage,respectively,and the rats in the western medicine group were given Progynova and Dydrogesterone by gavage.After 28 days of intervention,the ovarian tissues were selected for intermotility sampling,and the mRNA expression levels of apoptotic factors such as Bcl-2,Bax,Caspase-3 and p53 were measured by real-time quantitative polymerase chain reaction(RT-qPCR),and the protein expression levels of nuclear factor kappa B(NF-κB)p65 in ovarian tissue,estrogen receptor(ER)αand progesterone receptor(PR)in endometrium were measured by Western Blot.Results Compared with the normal group,the mRNA expression level of Bcl-2 in ovarian tissue was decreased,the mRNA expression levels of Bax,Caspase-3 and p53 were increased,Bcl-2/Bax ratio was decreased,the protein expression levels of NF-κB p65 in ovarian tissue and ERαand PR in endometrium in the model group were decreased(all P<0.05);compared with the model group,the mRNA expression level of Bcl-2 in ovarian tissue in the Chinese herbal medicine group was increased,the mRNA expression levels of Bax,Caspase-3 and p53 were decreased,Bcl-2/Bax ratio was increased,the protein expression levels of NF-κB p65 in ovarian tissue and ERαand PR in endometrium were increased(all P<0.05).Conclusion Xianzi Yizhen Capsules has effecs in improving the tripterygium glycosides-induced DOR in rats through enhancing ovarian reserv

关 键 词：仙子益真胶囊 卵巢储备功能减退 子宫内膜容受性 凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]