基于DNA条形码的武夷光唇鱼物种有效性  

Species validity of Acrossocheilus wuyiensis based on DNA barcode

作　　者：王荣达 张琰 郝信鹏 杨金权[1,2] WANG Rongda;ZHANG Yan;HAO Xinpeng;YANG Jinquan(Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学海洋动物系统分类与进化上海高校重点实验室,上海201306 [2]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2022年第4期858-864,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31872207)。

摘　　要：以线粒体COⅠ基因全序列为分子标记分析中国台湾省分布的台湾光唇鱼(Acrossocheilus paradoxus)和闽江分布的武夷光唇鱼(A.wuyiensis)的物种有效性。光唇鱼属(Acrossocheilus)6个种76尾样本的COⅠ基因全序列共获得21个单倍型,采用最大简约法(MP)、最大似然法(ML)和贝叶斯法(BI)构建的系统发育树表明,台湾光唇鱼和武夷光唇鱼构成单系群。K 2-P遗传距离分析显示台湾光唇鱼与武夷光唇鱼之间平均遗传距离仅为0.853%,远小于COⅠ条形码在物种间的2%遗传距离阈值。以ASAP物种界定法分析结果显示台湾光唇鱼和武夷光唇鱼为同一物种。因此,闽江分布的武夷光唇鱼与台湾光唇鱼应为同一物种,武夷光唇鱼为台湾光唇鱼的同物异名。Previous studies have shown that Acrossocheilus paradoxus from Taiwan and A.wuyiensis from Minjiang River are closely related species,and they are difficult to distinguish in morphological characteristics.Therefore,the validity of the two species is still controversial.In the present study,completemitochondrial COⅠ gene sequences were used to investigate the species validity of A.paradoxus and A.wuyiensis.A total of 21 haplotypes were obtained from 76 samples of 6 species in the genus.Phylogenetic trees constructed by maximum parsimony(MP),maximum likelihood(ML) and Bayesian(BI) methods showed that the two species formed a monophyletic group.Genetic distance analysis showed that the average Kimura 2-parameter distance between A.paradoxu s and A.wuyiensis was only 0.853%,far less than the 2% genetic distance threshold of DNA barcode among species.The ASAP method we also used to build species partitions,the result shows the two species were classified as the same species.Therefore,A.wuyiensis distributed in Minjiang River and A.paradoxus distributed in Taiwan should be a same species,and A.wuyiensis is the synonym of A.paradoxus.

关 键 词：台湾光唇鱼 线粒体COⅠ基因 同物异名 ASAP法 物种界定 

分 类 号：S917[农业科学—水产科学]