黑素瘤缺乏因子2和线粒体DNA在银屑病皮损和血清中的表达水平测定  

Detection of AIM2 in skin lesions and mtDNA in serum of psoriasis patients

作　　者：梁黛雯 王丽雅 赵莎 王小康 朱晓芳 朱国强[4] LIANG Dai-wen;WANG Li-ya;ZHAO Sha(The Second Xiangya Hospital of Central South University,Changsha 4100ll,China)

机构地区：[1]中南大学湘雅二医院,长沙410011 [2]扬州大学医学院 [3]扬州大学临床医学院皮肤科 [4]扬州大学兽医学院

出　　处：《实用皮肤病学杂志》2022年第3期147-150,共4页Journal of Practical Dermatology

基　　金：扬州市科技计划项目-社会发展(YZ2018065)。

摘　　要：目的检测寻常性银屑病患者皮损组织中黑素瘤缺乏因子2(AIM2)表达水平和血清中线粒体DNA(mtDNA)表达水平:观察poly(dA:dT)转染HaCaT细胞对HaCaT细胞增殖以及AIM2表达的影响,初步探讨AIM2和mtDNA在银屑病发病机制中的作用。方法实时荧光定量聚合酶链反应(qRT-PCR)检测寻常性银屑病患者组(PSO组)与健康对照组(CON组)血清中mtDNAmRNA表达水平:Western印迹(Western-blot)检测PSO和CON组皮肤组织中AIM2蛋白水平的表达差异:CCK-8法检测不同浓度的poly(dA:dT)转染HaCaT细胞增殖的情况:qRT-PCR法检测各组细胞中AIM2 mRNA的表达情况。结果qRT-PCR检测示PSO组血清中mtDNA mRNA表达水平明显升高,与CON组相比差异有统计学意义(P<0.0001);Westerm印迹示,PSO组皮损中AIM2蛋白表达水平明显升高,与CON组相比差异有统计学意义(P<0.001);细胞CCK8增殖实验示2μg/ml poly(dA:dT)及5μg/ml poly(dA:dT)处理组细胞增殖能力升高,其中5μg/ml poly(dA:dT)处理组细胞增殖能力提升明显,且差异有统计学意义(P<0.001);细胞qRT-PCR检测示2μg/ml及5μg/ml poly(dA:dT)处理组AIM2 mRNA的表达水平较对照组升高,且差异具有统计学意义(P<0.05,P<0.0001)。结论AIM2在银屑病患者皮损中高表达,AIM2内源性诱导因子mtDNA在银屑病患者血清中升高,且体外试验诱导AIM2升高可促进HaCaT细胞增殖,或提示AIM2可能参与了银屑病的发病。Objective To detect the expression levels of absent in melanoma 2(AIM2)in the skin lesions and Mitochondrial DNA(mtDNA)in serum of patients with psoriasis vulgaris.To observe the efect of the proliferation and AIM2 expression of HaCaT cells transfected with poly(dA:dT),and to preliminarily explore the role of AIM2 and mtDNA in the pathogenesis of psoriasis.Methods Quantitative real time polymerase chain reaction(qRT-PCR)method was used to detect the expression level of mtDNA mRNA in the serum of the psoriasis vulgaris group(PSO group)and the control group(CON group).The expression of AIM2 protein in skin tssues of PSO group and CON group was detected by Westerm-blot.Cell Counting Kit-8(CCK-8)assay was used to detect the proliferation of HaCaT cells transfered by poly(dA:dT)of different concentrations.The expression levels of AIM2 mRNA in each group were detected by qRT-PCR.Results The qRT-PCR results showed that the expression levels of mtDNA mRNA in serum of PSO group patients were significantly higher than the CON group,and the difference between groups was statistically significant(P<0.0001).Westerm blot analysis showed that AIM2 protein expression level in skin lesions of psoriasis patients was significantly higher than the CON group,and the difference between groups was statistical significant(P<0.001).CCK8 cell viability assay showed that cell proliferation ability of 2μg/ml poly(dA:dT)treatment group and 5 ug/ml poly(dA:dT)treatment group was increased.Compared with untreated group,cell proliferation ability of 5 ug/ml poly(dA:dT)treatment group was significantly improved,and the difference was statistically significant(P<0.001).qRT{PCR results showed that the expression levels of AIM2 mRNA in 2μg/ml Poly(dA:dT)group and 5ug/ml poly(dA:dT)group were higher than that in untreated group,and the diference was stastically significant(P<0.05,P<0.0001,respectively).Conclusion AIM2 is highly exressed in the skin lesions of patients with psoriasis.mtDNA is elevated in the serum of patients with psoriasis vulgari

关 键 词：银屑病 寻常性 黑素瘤缺乏因子2 线粒体DNA 炎症小体 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]