细菌代谢酶响应型荧光探针研究进展  

Enzyme-responsive fluorescent probes for bacteria imaging

作　　者：魏娆 胡海宇 张蕾磊 WEI Rao;HU Hai-yu;ZHANG Lei-lei(Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,活性物质发现与适药化研究北京市重点实验室,北京100050

出　　处：《药学学报》2022年第8期2313-2326,共14页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(22122705).

摘　　要：荧光探针是潜在的荧光团,可根据生物微环境变化、与被分析物相互作用或特定化学反应显示荧光信号。细菌代谢酶在细菌生命活动中发挥着至关重要的作用,对维持和调节细菌功能有重要影响。细菌代谢酶响应型荧光探针以其他检测技术无法比拟的空间分辨率和灵敏度阐明了细菌体内的复杂动力学过程。本篇综述回顾了细菌代谢酶响应型荧光探针的最新进展,依据酶分类系统进行整理,重点关注荧光团掩蔽策略、代谢酶响应模式以及相关生物成像应用。Fluorescent probes are potential fluorophores that display signals based on the changes in tissue microenvironment,interactions with analytes or specific biochemical reactions.Metabolic enzymes are the most important protein involved in bacteria activities.Complex dynamics of biological processes in bacteria are elucidated by these metabolic enzymes-based fluorescent probes with high spatial resolution and sensitivity.Here,we review recent advances in metabolic enzyme-responsive fluorescent probes for bacteria imaging.It was organized according to enzyme classification systems,focused on fluorescence masking strategies,molecular mechanisms of enzyme activation,and bio-related applications.

关 键 词：代谢酶 细菌 荧光探针 生物成像 探针设计 

分 类 号：R917[医药卫生—药物分析学]