鹿茸草水提取物中的单萜苷类成分  

Monoterpene glucosides from an aqueous extract of Monochasma savatieri

作　　者：刘琛 朱承根 徐成博 穆滨 郭庆兰 石建功 LIU Chen;ZHU Cheng-gen;XU Cheng-bo;MU Bin;GUO Qing-lan;SHI Jian-gong(Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China;Harbin Kanglong Pharmaceutical Co.,Ltd.,Harbin 150025,China)

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050 [2]哈尔滨市康隆药业有限责任公司,黑龙江哈尔滨150025

出　　处：《药学学报》2022年第8期2405-2415,共11页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81630094).

摘　　要：通过大孔吸附树脂、MCI树脂、Sephadex LH-20和HW-40C柱色谱,结合制备薄层色谱、反相HPLC和Flash等色谱分离技术,从鹿茸草水煎煮提取物中分离得到11个单萜苷类化合物。借助波谱数据解析、酸或酶水解以及电子圆二色谱(ECD)和NMR计算综合分析,确定了它们的结构(1~11),包括6个新结构化合物,分别命名为鹿茸草苷A~D(1~4)、G和H(7和8)。通过9-羟基芳樟醇3-O-β-D-葡萄糖苷(5)、(6Z)-9-羟基芳樟醇-3-O-β-D-葡萄糖苷(6)和kankanoside D1(9)的文献报道数据与本实验数据比较,首次确证了6和9的绝对构型。另外两个化合物分别鉴定为8-羟基香叶醇1-O-β-D-葡萄糖苷(10)和8-羟基香叶醇8-O-β-D-葡萄糖苷(11)。Eleven monoterpene glucosides were isolated from a water decoction of Monochasma savatieri by column chromatography over macroporous adsorbent resin,MCI resin,Sephadex LH-20,and HW-40C,combined with preparative TLC,reversed phase HPLC,and flash column chromatographic techniques.Their structures were elucidated by comprehensive analysis of spectroscopic data,along with acidic and enzymatic hydrolysis as well as electronic circular dichroism(ECD)and NMR calculations,including six new compounds(1-4,7 and 8),named monochasides A-D,G and H,respectively.Comparing the reported data of 9-hydroxylinaloyl 3-O-β-D-glucoside(5),(6Z)-9-hydroxylinaloyl 3-O-β-D-glucoside(6),and kankanoside D1(9)with those obtained in this study,the absolute configurations of 6 and 9 were proved for the first time.Other two compounds were identified as 8-hydroxygeraniol 1-O-β-D-glucopyranoside(10)and 8-hydroxygeraniol 8-O-β-D-glucopyranoside(11),respectively.

关 键 词：玄参科 鹿茸草 水提取物 单萜葡萄糖苷 鹿茸草苷 

分 类 号：R284[医药卫生—中药学]