巴戟天寡糖通过腺苷酸活化蛋白激酶信号通路改善去势大鼠缺血再灌注损伤心肌能量代谢的研究  

Energy metabolism of myocardium with ischemia-reperfusion injury in ovariectomized rats improved by morinda officinalis oligose through AMP-activated protein kinase pathways

作　　者：王志红[1] 黄冬梅[1] 崔金全[1] 程亮星[2] 吴成稳[3] Wang Zhihong;Huang Dongmei;Cui Jinquan;Cheng Liangxing(Department of Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014,China;Office of Academic Research,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院妇科,450014 [2]郑州大学第一附属医院科研处,450052 [3]郑州大学第二附属医院心外科,450014

出　　处：《中华实验外科杂志》2022年第7期1333-1336,共4页Chinese Journal of Experimental Surgery

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20200420)。

摘　　要：目的探讨腺苷酸活化蛋白激酶(AMPK)信号通路在巴戟天寡糖(MOO)改善去势大鼠缺血再灌注损伤(IRI)心肌能量代谢中的作用。方法100只健康雌性SD大鼠按随机数字表法均分为空白对照组、假IRI组、IRI组、MOO 2.1 g/(kg·d)组、MOO 0.7 g/(kg·d)组等5组,每组20只。后3组均按去势大鼠心肌IRI模型处理,其中MOO 2.1 g/(kg·d)组、MOO 0.7 g/(kg·d)组按体质量10 ml/kg用相应浓度MOO灌胃。结束后计算心肌梗死率,检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)水平,检测心肌组织磷酸化AMPK(p-AMPK)、沉默信息调节因子1(SIRT1)、过氧化物酶体增生物激活受体γ共激活因子1α(PGC-1α)蛋白。多组之间比较采用单因素方差分析,两两比较采用LSD-t检验。结果MOO 2.1 g/(kg·d)组、MOO 0.7 g/(kg·d)组心肌梗死率、血清CK-MB水平、血清LDH水平低于IRI组[心肌梗死率:(26.68±2.29)%、(33.30±3.27)%比(46.30±4.41)%,血清CK-MB水平:(100.01±6.58)U/L、(132.43±8.51)U/L比(173.19±12.92)U/L,血清LDH水平:(137.05±7.01)U/L、(210.76±11.84)U/L比(277.28±21.95)U/L,P<0.01];MOO 0.7 g/(kg·d)组心肌梗死率、血清CK-MB水平、血清LDH水平高于MOO 2.1 g/(kg·d)组[(33.30±3.27)%比(26.68±2.29)%,(132.43±8.51)U/L比(100.01±6.58)U/L,(210.76±11.84)U/L比(137.05±7.01)U/L,P<0.01]。MOO 2.1 g/(kg·d)组、MOO 0.7 g/(kg·d)组p-AMPK、SIRT1、PGC-1α相对表达量高于IRI组(p-AMPK:2.767±0.049、2.071±0.074比1.700±0.043,SIRT1:1.440±0.135、1.114±0.093比0.887±0.110,PGC-1α:1.024±0.113、0.905±0.075比0.639±0.067,P<0.01);MOO 0.7 g/(kg·d)组p-AMPK、SIRT1、PGC-1α相对表达量低于MOO 2.1 g/(kg·d)组(2.071±0.074比2.767±0.049,1.114±0.093比1.440±0.135,0.905±0.075比1.024±0.113,P<0.01)。结论MOO能够通过激活AMPK信号通路改善IRI心肌细胞能量代谢,减轻去势大鼠心肌IRI。Objective To investigate the role of AMP-activated protein kinase pathways(AMPK)in morinda officinalis oligose(MOO)improving energy metabolism of myocardium with ischemia-reperfusion injury(IRI)in ovariectomized rats.Methods A total of 100 female SD rats were randomly divided into the blank control group(n=20),the sham IRI group(n=20),the IRI group(n=20),the MOO 2.1 g/(kg·d)group(n=20)and the MOO 0.7 g/(kg·d)group(n=20).The rats in the last other three groups were made into myocardial IRI models.Before the operation,the rats of the two MOO groups were given homologous concentration MOO 10 ml/kg.The incidence of myocardial infarction was calculated,serum creatine kinase isoenzyme(CK-MB)and lactate dehydrogenase(LDH)levels were detected,and AMPK,silent information regulation 1(SIRT1),peroxisome proliferator activated receptorγco-activator 1α(PGC-1α)protein in the myocardium were detected too.One-way ANOVA was used for comparison among groups,and LSD-t test was used for comparison between the two groups.Results The incidences of myocardial infarction,the serum CK-MB levels,the serum LDH levels in the MOO 2.1 g/(kg·d)group and the MOO 0.7 g/(kg·d)group were lower than those in the IRI group[incidences of myocardial infarction:(26.68±2.29)%,(33.30±3.27)%vs.(46.30±4.41)%;serum CK-MB levels:(100.01±6.58)U/L,(132.43±8.51)U/L vs.(173.19±12.92)U/L;serum LDH levels:(137.05±7.01)U/L,(210.76±11.84)U/L vs.(277.28±21.95)U/L,P<0.01].The incidences of myocardial infarction,the serum CK-MB levels,the serum LDH levels in the MOO 0.7 g/(kg·d)group were higher than those in the MOO 2.1 g/(kg·d)group[(33.30±3.27)%vs.(26.68±2.29)%,(132.43±8.51)U/L vs.(100.01±6.58)U/L,(210.76±11.84)U/L vs.(137.05±7.01)U/L,P<0.01].The relative expression levels of AMPK,SIRT1,PGC-1αin the MOO 2.1 g/(kg·d)group and the MOO 0.7 g/(kg·d)were higher than those in the IRI group(p-AMPK:2.767±0.049,2.071±0.074 vs.1.700±0.043;SIRT1:1.440±0.135,1.114±0.093 vs.0.887±0.110;PGC-1α:1.024±0.113,0.905±0.075 vs.0.639±0.067,P<0.01).The

关 键 词：巴戟天寡糖 心肌缺血再灌注损伤 腺苷酸活化蛋白激酶 沉默信息调节因子1 过氧化物酶体增生物激活受体γ共激活因子1α 

分 类 号：R285.5[医药卫生—中药学]