山绿茶中1个新的三萜酸成分绝对构型的确定  

Determination of absolute configuration of a new triterpenic acid in leaves of Ilex hainanensis

作　　者：李新亮 符胜男 汤书婉 马莉 孙捷 李存玉[1,2] 郑云枫 彭国平[1,2] LI Xin-liang;FU Sheng-nan;TANG Shu-wan;MA Li;SUN Jie;LI Cun-yu;ZHENG Yun-feng;PENG Guo-ping(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Cllaborative Innouation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中国中药杂志》2022年第15期4084-4088,共5页China Journal of Chinese Materia Medica

摘　　要：研究山绿茶三萜酸类成分。采用碱水提取、大孔树脂吸附、制备高效液相色谱等方法分离纯化山绿茶中三萜酸类化学成分,依据理化性质、MS、NMR波谱数据、文献对比等方式进行结构鉴定,发现1个新的三萜酸类化合物:(3S,4R,5R,8R,9R,10R,14S,17S,18S,19R)-3,19-dihydroxyursa-12,20(30)-diene-24,28-dioic acid,并命名为ilexhainanin F。依据其结构特征,利用F-NMR的Mosher法对其绝对构型进行研究,通过比较Mosher酯的F-NMR化学位移,确定该化合物3位手性中心的绝对构型为S构型。This study aimed to explore the triterpenic acid components in leaves of Ilex hainanensis. Alkaline water extraction, macroporous resin adsorption, and high performance liquid chromatography were used to separate and purify the triterpenic acid components in leaves of I. hainanensis. The physical and chemical property analysis, MS, NMR spectroscopy, and literature comparison were performed to identify the structures, and a new triterpene acid compound was discovered:(3S, 4R, 5R, 8R, 9R, 10R, 14S, 17S, 18S, 19R)-3,19-dihydroxyursa-12,20(30)-diene-24,28-dioic-acid, and named ilexhainanin F. In addition, according to its structural characteristics, theF-NMR Mosher method was further employed to study its absolute configuration. By comparison of theF-NMR chemical shifts of Mosher esters, it was determined that the absolute configuration of the 3-position chiral center of the compound was the S configuration.

关 键 词：山绿茶 三萜酸 19F-NMR Mosher法 绝对构型 

分 类 号：R284[医药卫生—中药学]