外翻肠囊法研究隔山消提取物在正常和功能性消化不良模型大鼠中的肠吸收差异  

Intestinal absorption characteristics of root tuber of Cynanchum auriculatum extract in normal and functional dyspepsia model rats via everted intestine sac model

作　　者：刘利琴 张静雅 陆苑[1] 刘春花[3] 勾健 李竹珺 王爱民[3] 孙佳[1] LIU Li-qin;ZHANG Jing-ya;LU Yuan;LIU Chun-hua;GOU Jian;LI Zhu-jun;WANG Ai-min;SUN Jia(State Key Laboratory of Functions and Applications of Medicinal Plants,Provincial Key Laboratory of Pharmaceutics in Guizhou Province,Guizhou Medical Universily,Guiyang 550004,China;Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Guizhou Medical University,Guiyang 550004,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《中国中药杂志》2022年第14期3915-3922,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81760700,U1812403);贵州医科大学博士启动基金项目(校博合J字[2020]013号)。

摘　　要：考察隔山消提取物在正常与功能性消化不良(FD)模型大鼠病理状态下的肠吸收特性差异,采用离体外翻肠囊模型,通过UPLC-MS/MS检测隔山消提取物中丁香酸、东莨菪内酯、告达亭、白首乌二苯酮、青阳参苷元、去酰基萝藦苷元6个代表性成分在不同肠段的含量,计算累积吸收量(Q)和吸收速率常数(K)。结果发现,隔山消提取物中6个成分均可吸收进入肠囊,且未呈饱和状态,推测东莨菪内酯的吸收方式可能有主动转运,而其余成分的吸收方式可能为被动扩散。正常状态下,丁香酸、白首乌二苯酮在回肠吸收较好,青阳参苷元、去酰基萝藦苷元在回肠、十二指肠吸收较好,告达亭在结肠吸收较好,而东莨菪内酯低、中、高剂量分别在空肠、回肠、结肠吸收最好。而模型状态下各成分最佳吸收部位均在回肠。各成分不同剂量下正常组与模型组的吸收特征较为复杂,未表现出一致的吸收趋势,提示小肠对药物的吸收具有选择性,且FD病理状态会改变药物的吸收特征。该研究可为隔山消的临床用药和产品开发提供理论依据。The study investigated the difference of intestinal absorption characteristics of root tuber of Cynanchum auriculatum extract between normal and functional dyspepsia(FD) model rats with everted intestine sac model.The content of syringic acid, scopoletin, caudatin, baishouwu benzophenone, qingyangshengenin and deacyhmetaplexigenin in the C.auriculatum extract in different intestinal segments was detected by UPLC-MS/MS.The cumulative absorption amount(Q) and absorption rate constant(K) of the six chemical constituents were calculated.The results showed that the six components could be absorbed into the intestinal sac and were unsaturated, which indicated that the absorption mechanism of scopoletin was active transport in the intestine, while that of the other five components were passive diffusion.For normal group, the syringic acid and baishouwu benzophenone in ileum, qingyangshengenin and deacyhmetaplexigenin in ileum and duodenum, and caudatin in colon were well absorbed and scopoletin at low, medium and high concentrations was found excellent absorption in jejunum, ileum, and colon, respectively.Whereas the best absorption site of each component was ileum in model group.The absorption characteristics of each component between normal group and model group were complex at different concentrations, showing inconsistent tendency of absorption, which suggested that the components of root tuber of C.auriculatum extract were selectively absorbed in small intestine, and the absorption characteristics of the six components could be changed under FD status.This study provided theoretical basis for the clinical drug application and development of root tuber of C.auriculatum.

关 键 词：隔山消提取物 功能性消化不良 离体外翻肠囊模型 肠吸收 

分 类 号：R285.5[医药卫生—中药学]