不同处理下枳β-淀粉酶基因家族PtrBAM2~PtrBAM8的表达分析  

Expression Analysis of Seven β-Amylase Encoding Gene PtrBAM2~PtrBA-M8 in Poncirus trifoliata under Various Treatments

作　　者：吴昱 彭婷[1] Wu Yu;Peng Ting(National Navel Orange Research Center,Gannan Normal University,Ganzhou,341000)

机构地区：[1]赣南师范大学,国家脐橙工程技术研究中心,赣州341000

出　　处：《分子植物育种》2022年第13期4282-4288,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31760563)资助。

摘　　要：前期研究克隆了枳(Poncirus trifoliata) β-淀粉酶家族的8个成员PtrBAM1~PtrBAM8,但仅对PtrBAM1进行了胁迫表达和抗寒功能分析,未对PtrBAM2~PtrBAM8展开研究。本研究利用实时荧光定量PCR检测了植株在4℃处理、200 mmol/L Na Cl处理、200 mmol/L麦芽糖处理、离体脱水处理和100μmol/L ABA处理后叶片PtrBAM2~PtrBAM8的相对表达量。结果表明:7个成员中PtrBAM2和PtrBAM4、PtrBAM5和PtrBAM6对不同非生物逆境处理的表达模式相似。4℃处理下,PtrBAM5和PtrBAM6的表达受到抑制,其他成员表达上调,PtrBAM2和PtrBAM4表达量上调最大;除PtrBAM3外,PtrBAM2-8在脱水处理下都表达上调;盐胁迫下,PtrBAM2和PtrBAM4表达上调,PtrBAM5和PtrBAM6表达下调。在外源ABA处理下,PtrBAM3表达下调,其余成员表达量都上调。外源麦芽糖处理下PtrBAM2~PtrBAM8的表达整体都呈上调趋势。因此,PtrBAM2~PtrBAM8可能在枳胁迫响应中扮演重要功能。In our previous study,eight members of the β-amylase encoding gene family of Poncirus trifoliata were cloned.Only the expression pattern and molecular function of PtrBAM1 was characterized,and no relevant studies were carried out with PtrBAM2~PtrBAM8.In this study,real-time quantitative PCR was used to detect the relative expressions of PtrBAM2~PtrBAM8 in leaves after 4 ℃,in vitro dehydration,200 mmol/L NaCl,100 μmol/L ABA and 200 mmol/L maltose treatments.The results showed that the expression patterns of PtrBAM2 and PtrBAM4,PtrBAM5 and PtrBAM6 were similar in response to different abiotic stress treatments.Under 4 ℃ treatment,the expressions of PtrBAM5 and 6 were depressed,and those of the other members were increased;except PtrBAM3,the expressions of PtrBAM2~PtrBAM8 were up-regulated under dehydration;Under salt stress,the expressions of PtrBAM2 and PtrBAM4 were up-regulated,and the expressions of PtrBAM5 and PtrBAM6 were down regulated.Under exogenous ABA treatment,the expression of PtrBAM3 was down-regulated and those of the other members were up-regulated.The expressions of PtrBAM2~PtrBAM8 were up-regulated under exogenous maltose application.Therefore,PtrBAM2~PtrBAM8 may play important roles in the stress response of Poncirus trifoliata.

关 键 词：柑橘 淀粉酶 基因家族 非生物胁迫 表达分析 

分 类 号：S666[农业科学—果树学]