基于高通量测序的蓝莓果实转录组SSR信息分析  

SSR Information Analysis of Blueberry Fruit Transcriptome Based on High Throughput Sequencing

作　　者：王小敏[1] 张春红[1] 胡丽超 吴文龙[1] 李维林 WANG Xiaomin;ZHANG Chunhong;HU Lichao;WU Wenlong;LI Weilin(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014;Academy of Forestry,Nanjing Forestry University/Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing,Jiangsu 210037)

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]南京林业大学林学院,南方现代林业协同创新中心,江苏南京210037

出　　处：《北方园艺》2022年第13期49-54,共6页Northern Horticulture

基　　金：中央财政林业科技示范推广资金资助项目(苏[2021]TG08)。

摘　　要：以蓝莓品种‘巴尔德温’(Vaccinium Spp.var.Baldwin)的果实为试材,通过转录组高通量测序获得Unigenes数据,分析转录组的SSR信息,开发蓝莓EST-SSR功能性标记引物,以期为开展蓝莓的基因定位及果实品质形成的分子生物学机制等研究提供参考依据。结果表明:转录组测序共获得73 468条Unigenes,其中SSR位点发生频率为20.81%,含有序列的SSR位点3 354条。蓝莓果实转录组SSRs的平均长度为20 bp,平均分布频率为1/3.90 kb。在蓝莓转录组SSR中,2个碱基重复比例最高(41.02%),其次为单碱基重复。单个SSR位点的蓝莓转录组SSR共包含173种重复基元,A/T(36.56%)和AG/CT(41.03%)分别为优势重复基元类型,含有SSR位点的Unigene被富集到3个Ontology类别的57个GO term中,其中涉及生殖、结合、多细胞生物过程的GO term最多。基于蓝莓果实转录组信息分析,最终设计了82对SSR序列引物,开发这些潜在多态性高的SSR在蓝莓遗传育种研究和资源评价上具有较高的应用价值。Taking the fruit of blueberry variety ‘Baldwin’ as the material, Unigenes was obtained by high-throughput sequencing, SSR information analysis was analyzed, and blueberry EST-SSR functional marker primers were developed, in order to provide reference for the molecular biological mechanism of gene mapping and fruit quality formation of blueberry.The results showed that, a total of 73 468 Unigenes were obtained by transcriptome sequencing, of which the frequency of SSR was 20.81%,and 3 354 SSR sites containing sequences, were obtained.The average length of transcriptome SSRs in blueberry fruit was 20 bp and the average distribution frequency was 1/3.90 kb.In the blueberry transcriptome SSR,the proportion of Dinucleotide was the highest(41.02%),followed by mononucleotide.The blueberry transcriptome SSR of a single SSR site contains 173 repeat nucleotides, and A/T(36.56%) and AG/CT(41.03%) were the dominant repeat unit types, respectively.Unigene containing SSR sites was enriched into 57 GO terms of three ontology categories, of which the most GO terms involved in reproduction, binding and multicellular biological processes.Based on the transcriptome information analysis of blueberry fruit, 82 pairs of SSR sequence primers were finally designed.The development of these potentially polymorphic SSRs might have high application value in blueberry genetic breeding research and resource evaluation.

关 键 词：蓝莓果实 转录组 SSR 引物设计 

分 类 号：S663.9[农业科学—果树学]