恶性血液病患者血小板HLA-Ⅰ抗体产生与HLA-A、B基因之间的相关性分析  

Correlative Analysis between Production of Platelet HLA-ⅠAntibody and HLA-A,B Genes in Patients with Malignant Hematological Diseases

作　　者：高晓云[1] 寇立舵 田华[1] 王新华[1] GAO Xiao-Yun;KOU Li-Duo;TIAN Hua;WANG Xin-Hua(Department of Blood Transfusion,Aerospace Center Hospital,Beijing 100049,China)

机构地区：[1]航天中心医院输血科,北京100049

出　　处：《中国实验血液学杂志》2022年第4期1203-1207,共5页Journal of Experimental Hematology

摘　　要：目的:探讨恶性血液病患者血小板HLA-Ⅰ抗体产生与HLA-A、B基因之间的相关性和血小板HLA-Ⅰ抗体产生的易感基因。方法:选择本院血液科需多次输注血小板治疗的恶性血液病患者作为研究对象,采用ELISA法对患者进行HLA-I类抗体检测,根据抗体检测结果将患者分为抗体阳性组和抗体阴性组,提取患者基因组DNA后进行HLA-A、B基因测序,比较两组之间HLA-A、B基因频率的差异。结果:患者HLA-I类抗体阳性率为22.95%。对100例研究对象进行HLA-A、B基因测序,共获得HLA-A等位基因13个,HLA-B等位基因14个,其中HLA-A*24、HLA-A*30、HLA-B*13等位基因频率在抗体阳性组与阴性组之间存在统计学差异(P<0.05)。抗体阳性组HLA-A*30、HLA-B*13等位基因频率低于抗体阴性组(RR=0.107,0.387),HLA-A*24等位基因频率高于抗体阴性组(RR=1.412)。HLA-A*24、HLA-A*30、HLA-B*13基因高分辨基因分型显示等位基因HLA-A*24∶02、HLA-A*30∶01、HLA-B*13∶02在抗体阳性组和阴性组间存在差异,RR值分别为1.412、0.107、0.125,95%CI分别为0.961-2.075、0.016-0.721、0.300-0.515。结论:恶性血液病患者HLA-A*24∶02等位基因可能是血小板HLA-Ⅰ抗体产生的易感基因,而HLA-A*30∶01、HLA-B*13∶02等位基因可能是保护基因。Objective:To investigate the correlation between the production of platelet HLA-Ⅰantibody and HLA-A,B genes in patients with malignant hematological diseases,and explore the susceptible gene for producing platelet HLA-Ⅰantibody.Methods:Patients with malignant hematological diseases who had received multiple platelet transfusion were selected as the research objects in the Department of Hematology of our hospital.Platelet HLA-I antibody were screened by ELISA,and the patients were divided into positive and negative groups according to the results.HLA-A and B genes were sequenced after genomic DNA was extracted,and the frequencies of them were compared between the two groups.Results:The positive rate of platelet HLA-I antibody was 22.95%.A total of 13 HLA-A alleles and 14 HLA-B alleles were obtained after the HLA-A and B genes sequencing in 100 cases.The frequencies of HLA-A*24,HLA-A*30,and HLA-B*13 were significantly different between the two groups(P<0.05).Frequencies of HLA-A*30 and HLA-B*13 in the positive group were lower than those in the negative group(RR=0.107,0.387),but HLA-A*24 was higher(RR=1.412).After high-resolution typing of HLA-A*24,HLA-A*30,and HLA-B*13,frequencies of HLA-A*24∶02,HLA-A*30∶01,and HLA-B*13∶02 were significantly different between the two groups,the RR value was 1.412,0.107,and 0.125,95%CI was 0.961-2.075,0.016-0.721,and 0.300-0.515,respectively.Conclusion:HLA-A*24∶02 may be a susceptible gene for producing platelet HLA-Ⅰantibody in patients with malignant hematological diseases,while HLA-A*30∶01 and HLA-B*13∶02 may be two protective genes.

关 键 词：恶性血液病 人类白细胞抗原 易感基因 血小板抗体 

分 类 号：R457.1[医药卫生—治疗学]