高效液相色谱法检测绞股蓝茶中的蔗糖含量  

Determination of Sucrose in Gynostemma pentaphyllum Tea by High Performance Liquid Chromatography

作　　者：马倩 杜雨 MA Qian;DU Yu(Ankang Municipality of Quality and Technical Inspection Test Center,Ankang 725000,China)

机构地区：[1]安康市质量技术检验检测中心,陕西安康725000

出　　处：《现代食品》2022年第16期200-202,206,共4页Modern Food

摘　　要：本试验利用高效液相色谱法检测绞股蓝茶中蔗糖的含量,样品经水超声提取,亚铁氰化钾和乙酸锌溶液去除蛋白等杂质后离心,通过0.22μm的滤膜过滤,用乙腈和水作为流动相,氨基色谱柱分离,示差折光检测器进行定性定量分析。该方法定量限为0.10 g/100 g,适合于绞股蓝茶中蔗糖的检测。In this study,reversed-phase high performance liquid chromatography was used to determine the content of sucrose in Gynostemma pentaphyllum tea.The samples were extracted by water ultrasonic,and the impurities such as protein were removed by potassium ferrocyanide and zinc acetate solution.After centrifugation,the samples were filtered by a 0.22μm filter membrane,and separated by an amino column using acetonitrile and water as mobile phase.Differential refractive index detector was used for qualitative and quantitative analysis.The quantitative limit of the method was 0.10 g/100 g,which was suitable for the detection of sucrose in Gynostemma pentaphyllum tea.

关 键 词：蔗糖 绞股蓝茶 高效液相色谱 

分 类 号：O657.7[理学—分析化学]