猪胚胎肝脏发育的形态学研究  

Morphological study on liver development of swine embryos

作　　者：张莉平[1] 孙雪婧 ZHANG Liping;SUN Xuejing(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学动物医学院,山东青岛266109

出　　处：《畜牧与兽医》2022年第8期45-50,共6页Animal Husbandry & Veterinary Medicine

基　　金：青岛农业大学校高层次人才科研基金(663/1120020)。

摘　　要：为了研究猪胚胎肝脏在不同发育时期的组织形态结构特点,试验选取6组平均胚体重分别为(2.3±0.2)g、(5.6±0.8)g、(29.6±4.2)g、(84.8±15.2)g、(500±26.5)g、(1250±50.1)g的猪胚胎,取其肝脏组织,制备石蜡切片后进行常规HE染色,用光学显微镜观察。结果发现,当猪胚胎生长到重量2.3 g时,肝脏血管的轮廓初步形成;在胚胎生长到重量为5.6 g时,肝组织中就已经发现了中央静脉雏形、此时肝板已经有了雏形;随着胚胎的成长,肝脏的窦状隙先增大后减小,造血灶先增多再减少,至猪胚胎重量为500 g时造血灶已观察不到,此时出现门管区结构;胚胎生长到1250 g时,肝小叶基本形成,但结缔组织较少,肝小叶之间分界仍不明显。本试验结果为猪胚胎时期肝脏的发育提供了组织形态学基础。In order to study the morphological structure of the liver in swine embryos at different developmental stages,swine embryos of(2.3±0.2)g,(5.6±0.8)g,(29.6±4.2)g,(84.8±15.2)g,(500±26.5)g and(1250±50.1)g swine embryos were selected.The paraffin sections of the liver tissues were obtained and stained with H-E and were observed under a light microscope.The results showed that the outline of the blood vessels was initially formed in the liver of the 2.3 g swine embryos.Embryonic central veins were found in the embryonic liver tissue,and the liver plate was formed in the 5.6 g swine embryos.With the development of the embryo,the sinusoids of the liver were enlarged and then decreased.The hematopoietic areas were increased and then decreased.In the 500 g swine embryos,the hemato⁃poietic foci were not observed and the portal area was formed.In the 1250 g swine embryos,the hepatic lobule was generally formed.The connective tissues in the liver were still scarce and the boundary between the hepatic lobules was still not obvious.This experiment provided histomorphologic basis for research on the development of liver in the embryonic stage of swine.

关 键 词：猪 肝脏 胚胎发育 组织结构 

分 类 号：S852.16[农业科学—基础兽医学]