α-倒捻子素对帕金森病小鼠的神经保护作用  

Neuroprotective effect ofα-mangostin on Parkinson's disease in mice

作　　者：庞洪波[1] 何晓非[1] 张译文 李琳琳[1] PANG Hongbo;HE Xiaofei;ZHANG Yiwen;LI Linlin(Department of Neurology,Suining Central Hospital,Suining,Sichuan 629000,China;Department of Neurology,Suining Chinese Medicine Hospital,Suining,Sichuan 629000,China)

机构地区：[1]遂宁市中心医院神经内科,四川遂宁629000 [2]遂宁市中医院神经内科,四川遂宁629000

出　　处：《安徽医药》2022年第9期1710-1714,I0001,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨α-倒捻子素对帕金森病小鼠的神经保护作用。方法2018年7月至2019年8月,将40只C57BL/6小鼠按随机数字表法分为四组,分别为对照组、模型组、α-倒捻子素给药组(低剂量组、高剂量组),每组10只。模型组与α-倒捻子素给药组使用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导帕金森病小鼠模型。α-倒捻子素给药组分别给予5 mg•kg^(−1)•d^(−1)、15 mg•kg^(−1)•d^(−1)的α-倒捻子素灌胃给药。给药后14 d,使用爬杆实验、转棒实验、悬挂实验观测小鼠的行为学表现;高压液相电化学技术测定纹状体多巴胺、高香草酸、3,4-二羟基苯乙酸(DOPAC)的含量;免疫荧光染色检测酪氨酸羟化酶(TH)表达;TUNEL染色检测细胞凋亡水平;蛋白质印迹法检测凋亡相关蛋白表达。结果与对照组(9.3±1.7)s、(205.8±31.3)s、(2.2±0.4)分、(2051.4±195.2)ng/g、(1677.3±128.0)ng/g、(395.8±41.1)ng/g、(1.00±0.17)、(1.00±0.14)、(1.00±0.15)比较,模型组小鼠的爬杆时间(19.8±3.5)s升高,转棒停留时间(118.9±28.4)s和悬挂实验得分(1.0±0.3)分均降低;纹状体多巴胺(911.3±88.5)ng/g、高香草酸(11190.5±83.3)ng/g、DOPAC(187.9±20.3)ng/g含量降低;黑质区TH表达降低;黑质区细胞凋亡水平升高;黑质区B细胞淋巴瘤-2(Bcl-2)(0.23±0.11)表达量降低,Bcl-2相关X蛋白(Bax)(3.15±0.42)和活化胱天蛋白酶-9(cleaved-caspase-9)(4.27±0.52)表达量升高(P<0.05)。与模型组比较,低剂量组和高剂量组小鼠的爬杆时间[(15.4±3.1)s、(11.9±2.6)s]降低,转棒停留时间[(166.7±19.9)s、(192.1±21.5)s]和悬挂实验得分[(1.5±0.4)分、(1.9±0.6)分]均升高;纹状体多巴胺[(1388.6±159.9)ng/g、(1728.2±180.6)ng/g]、高香草酸[(1366.7±109.2)ng/g、(1510.8±112.4)ng/g]、DOPAC[(266.5±33.7)ng/g、(320.3±40.4)ng/g]含量升高;黑质区TH表达升高;黑质区细胞凋亡水平降低;黑质区Bcl-2表达量[(0.44±0.19)、(0.94±0.22)]升高,Bax[(2.06±0.38)、(1.18±0.20)]和cleaveObjective To explore the neuroprotective effect ofα-mangostin on Parkinson's disease(PD)in mice.Methods The starting and ending time of this study was from July 2018 to August 2019.Forty C57BL/6 mice were randomly assigned into four groups:control group,model group,α-mangostin administration group(low dose group and high dose group),there were 10 rats in each group.1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)was used to induce Parkinson's disease model in the model group and theα-mangostin administration group.Theα-mangostin administration group was administered with 5 mg•kg^(−1)•d^(−1) and 15 mg•kg^(−1)•d^(−1) ofα-mangostin by gavage.On the 14th day after administration,the behavior of mice was observed by climbing bar test,rotating bar test and hanging test.The contents of dopamine,homovanillic acid and 3,4-dihydroxyphenylacetic acid(DOPAC)in striatum were mea-sured by high pressure liquid phase electrochemical technique.The expression of Tyrosine hydroxylase(TH)was detected by immu-nofluorescence staining;apoptosis level was detected by TUNEL staining.The expression of apoptosis-related protein was detected by Western blotting.Results Compared with the control group(9.3±1.7)s,(205.8±31.3)s,(2.2±0.4)points,(2051.4±195.2)ng/g,(1677.3±128.0)ng/g,(395.8±41.1)ng/g,(1.00±0.17),(1.00±0.14),(1.00±0.15),the climbing time(19.8±3.5)s of the mice in the model group was increased,the rod staying time(118.9±28.4)s and suspension test scores(1.0±0.3)points were decreased,the content of dopamine(911.3±88.5)ng/g,homovanillic acid,(11190.5±83.3)ng/g and DOPAC(187.9±20.3)ng/g in striatum were decreased,the expression of TH in substantia nigra was decreased,the level of cell apoptosis in substantia nigra was increased,the expression of B cell lymphoma-2(Bcl-2)(0.23±0.11)was decreased and the expression of Bcl2-Associated X protein(Bax)(3.15±0.42)and cleaved-cas-pase-9(4.27±0.52)were increased(P<0.05).Compared with the model group,the climbing time of the mice in the low-dose group and

关 键 词：山竹子 α-倒捻子素 帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 多巴胺 神经保护 小鼠 近交C57BL 

分 类 号：R285.5[医药卫生—中药学]