抑制荧光猝灭法测定痕量双酚S的研究  

Determination of trace bisphenol-S (BPS) by inhibitory fluorescence quenching method

作　　者：刘宝亮 姚成龙 翟翔熊 陈正玲 LIU Bao-liang;YAO Cheng-long;ZHAI Xiang-xiong;CHEN Zheng-ling(School of Chemical Engineering and Materials,Changzhou Institute of Technology,Changzhou 213032,China)

机构地区：[1]常州工学院化工与材料学院,江苏常州213032

出　　处：《现代化工》2022年第8期248-251,共4页Modern Chemical Industry

基　　金：江苏省高等学校自然科学研究面上项目(19KJB530004)。

摘　　要：建立了一种吖啶橙-Fenton试剂荧光光谱法测定双酚S含量的方法。在酸性介质中,吖啶橙能被Fenton试剂产生的羟自由基氧化并引起荧光猝灭,而双酚S能够抑制羟自由基对吖啶橙的氧化作用,其抑制荧光猝灭程度在一定范围内与BPS的浓度呈线性关系。研究结果表明,在最佳实验条件下,双酚S浓度在2.5×10^(-8)~1.5×10^(-7)mg/L范围内与吖啶橙荧光强度变化ΔF呈良好的线性关系(R=0.996 55),检出限为2.661×10^(-9)mg/L。该测定方法简便、快速、需样量少、灵敏度高,加标回收率为96.02%~101.4%。A new method is established for the determination of trace bisphenol-S based on the inhibition effect of bisphenol-S on the reaction between Fenton reagent and acridine orange.In sulfuric acid medium, acridine orange can be oxidized by hydroxyl radical produced by Fenton reagent, causing fluorescence quenching, while bisphenol S can inhibit the oxidation of hydroxyl radical on acridine orange.There is a linear relationship between the inhibitory effect on fluorescence quenching and the concentration of bisphenol-S within a certain range.Experimental results show that there exists a good relationship(R=0.996 55) between the concentration of bisphenol S in the range of 2.5×10^(-8)mg·L-1.5×10^(-7)mol·Land the change of acridine orange fluorescence intensity(ΔF) under the optimum conditions, the detection limit is 2.661×10^(-9)mg·L,and the recoveries are between 96.02%-101.4%.This method is fast, simple and high selectivity, and need few amount of samples.

关 键 词：双酚S 吖啶橙 FENTON试剂 荧光分光光度法 

分 类 号：O657.3[理学—分析化学]