脱氮除磷功能菌泥强化低溶解氧ACF-BAF工艺处理猪场沼液效能及微生物种群分析  

The performance and microbial community structure of a bioaugmentated ACF-BAF process treating anaerobic digested swine wastewater under low dissolved oxygen condition

作　　者：刘思强 信欣[1] 朱羽蒙 叶芝祥[1] 羊依金[1] 郑启莲 罗竣怀 刘嘉丽 LIU Siqiang;XIN Xin;ZHU Yumeng;YE Zhixiang;YANG Yijin;ZHENG Qilian;LUO Junhuai;LIU Jiali(College of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225,China)

机构地区：[1]成都信息工程大学资源环境学院,成都610225

出　　处：《环境工程学报》2022年第7期2397-2407,共11页Chinese Journal of Environmental Engineering

基　　金：成都市科技局技术创新研发项目(2022-YF05-00811-SN);四川省科技计划项目(2021YFG0267);2021年大学生创新创业训练计划项目(202110621050)。

摘　　要：猪场沼液是一种高氨氮低C/N比废水,传统工艺处理时启动时间长、运行管理不便、能耗高、总氮难去除且除磷效果差。本研究以活性炭纤维(activated carbon fiber,ACF)作为曝气生物滤池(biological aerated filter,BAF)的滤料,接种特定的脱氮除磷菌泥,在低溶解氧(DO=(0.6±0.1)mg·L^(-1))条件下,通过改变进水NH_(4)^(+)-N质量浓度的方式在100 d内成功启动了处理猪场沼液的生物强化ACF-BAF,对其微生物种群组成和功能菌群活性进行了分析,并探讨了其脱氮除磷的机理。结果表明,ACF-BAF启动成功后的NH_(4)^(+)-N、TN、TP和COD的平均去除率分别为61.03%、51.87%、52.58%和77.11%,其对氮、磷的去除负荷分别为0.1430 kg·(m^(3)·d)^(-1)和0.0093 kg·(m^(3)·d)^(-1),均显著高于传统工艺及部分新型工艺。其中NH_(4)^(+)-N、TP和COD的去除过程符合一级动力学方程,TN的去除过程符合二级动力学方程。高通量测序结果表明,处理猪场沼液的ACF-BAF反应系统中存在的与脱氮除磷功能相关菌属有Candidatus_Brocadia(2.22%)、反硝化菌norank_f__NS9_marine_group(4.02%)、Acinetobacter(8.34%)和除磷菌Micropruina(6.09%),norank_f__PHOS-HE36(2.36%)。ACF-BAF系统中脱氮过程以厌氧氨氧化和反硝化途径为主,除磷过程主要为微生物聚磷。将ACF-BAF工艺应用于实际猪场沼液处理,可快速启动并实现高效同步脱氮除磷,节约后续处理成本。Anaerobic digested swine wastewater is a kind of wastewater with high ammonia nitrogen and low C/N ratio,the traditional treatment process has the disadvantages of long start-up time,inconvenient operation and management,high energy consumption,difficult removal of total nitrogen and poor phosphorus removal.In this study,activated carbon fiber(ACF)was used as the filter media of biological aerated filter(BAF),inoculated with specific nitrogen and phosphorus removal bacterial sludge.Under low dissolved oxygen(DO=(0.6±0.1)mg·L^(-1))conditions,the bioaugmented ACF-BAF was successfully started within 100 d by changing the influent NH_(4)^(+)-N concentration,and the microbial population composition and functional bacterial activity were analyzed,and the mechanism of nitrogen and phosphorus removal was investigated.The results indicated that the average removal rates of NH_(4)^(+)-N,TN,TP and COD were 61.03%,51.87%,52.58%,and 77.11%,respectively.The removal loads of nitrogen and phosphorus were 0.1430 kg·(m^(3)·d)^(-1)和0.0093 kg·(m^(3)·d)^(-1),respectively,which were significantly higher than those of the conventional process and some new processes.The removal process of NH_(4)^(+)-N,TP and COD could be fitted by the first-order kinetic model,and the TN removal process could be fitted by the second-order kinetic model equation.The results of high-throughput sequencing showed that the bacteria related to nitrogen and phosphorus removal in the ACF-BAF system were Candidatus_Brocadia(2.22%),norank_f__NS9_marine_group(4.02%),Acinetobacter(8.34%),Micropruina(6.09%),norank_f__PHOS-HE36(2.36%).The results of activity and contribution rate of bacteria showed that nitrogen removal process in ACF-BAF was realized mainly by anaerobic ammonia oxidation and denitrification,and phosphorus removal process in ACF-BAF was realized mainly microbial phosphorus accumulation.The application of ACF-BAF process treating anaerobic digester liquor of real swine wastewater can realize quick start-up and realize an efficient simultaneous

关 键 词：猪场沼液 活性炭纤维(ACF) 曝气生物滤池(BAF) 生物强化 同步脱氮除磷 微生物种群 

分 类 号：X703.1[环境科学与工程—环境工程]