传染性囊病病毒对IgM^(+)TIM4^(+)B细胞分化的影响  

Effects of infectious bursal disease virus on the differentiation of IgM^(+)TIM4^(+)B cells

作　　者：何雪阳 肖星星[1] 燕苗苗 杨顺利[1] 张辛铭 蔡建平[1,2] HE Xue-yang;XIAO Xing-xing;YAN Miao-miao;YANG Shun-li;ZHANG Xin-ming;CAI Jian-ping(State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2022年第7期908-914,共7页Chinese Veterinary Science

基　　金：中国农业科学院创新工程项目(家畜疫病病原生物学国家重点实验室(中国农业科学院兰州兽医研究所)开放课题基金)(CAAS-ASTIP-2014-LVRI-09)。

摘　　要：以佛波酯(PMA)于体外培养中刺激原代法氏囊细胞(PBCs)诱导产生的IgM^(+)TIM4^(+)B细胞为模型,分析传染性囊病病毒(IBDV)感染对IgM^(+)TIM4^(+)B细胞分化形成及IBDV在IgM^(+)TIM4^(+)B和IgM^(+)TIM4^(-)B细胞中的复制动态。结果显示,PMA刺激后第24~48小时PBCs中IgM^(+)TIM4^(+)B细胞比例相对稳定;而以IBDV感染经PMA刺激24 h的PBCs后,IgM^(+)TIM4^(+)B细胞比例显著减少且与IBDV感染剂量成负相关,IgM^(+)TIM4^(+)B细胞的IBDV-VP1 RNA水平也显著低于IgM^(+)TIM4^(-)B细胞。结果提示,IBDV在IgM+TIM4+B细胞和IgM^(+)TIM4^(-)B细胞中的复制过程存在差异。Here we developed a cellular model in vitro of IgM^(+)TIM4^(+)B cell induced from the primary bursa cells(PBCs)by the phorbol myristate acetate(PMA)treatment for probing the impacts of infectious bursal disease virus(IBDV)on the formation of IgM^(+)TIM4^(+)B cells,and the replications of IBDV in this novel B cell subset.The results revealed that an unfluctuating proportion of IgM^(+)TIM4^(+)B cells could be induced in PBCs during the 24—48 hours after PMA stimulation.However,the PBCs treated with PMA for 24 h were infected with IBDV,the ratio of IgM^(+)TIM4^(+)B cells was significantly reduced and negatively correlated with the infection dose of IBDV,and simultaneously the RNA level of IBDV-VP1 in IgM^(+)TIM4^(+)B cells was significantly decreased compared to that of IgM^(+)TIM4^(-)B cells.These results suggested the differences in IBDV replication in IgM^(+)TIM4^(+)B cells and IgM^(+)TIM4^(-)B cells.

关 键 词：鸡传染性囊病病毒 原代法氏囊细胞 鸡TIM4 IgM^(+)TIM4^(+)B细胞 

分 类 号：S852.659.4[农业科学—基础兽医学]