大鼠角膜ATR-FTIR光谱变化与死亡时间的关系  

Relationship between PMI and ATR-FTIR spectral changes of Rat’s cornea

作　　者：吴慧 刘芳芳 伍俊达 谢英[1] Wu Hui;Liu Fangfang;Wu Junda;Xie Ying(Deparment of Forensic Medicine,North Sichuan Medical College,Nanchong,Sichuan,637000,China;Ybin City Public Security Bureau(Xuzhou District Branch),Yibin,Sichuan,644000,China)

机构地区：[1]川北医学院法医学系,四川南充637000 [2]宜宾市公安局叙州区分局,四川宜宾644000

出　　处：《中国法医学杂志》2022年第4期369-373,共5页Chinese Journal of Forensic Medicine

基　　金：四川省基层卫生事业发展研究中心课题(SWFZ17-Z-12);四川省教育厅基金项目(15ZA0215);南充市市校合作科研专项资金项目(19SXHZ0197)。

摘　　要：目的 应用衰减全反射傅里叶变换红外(ATR-FTIR)光谱技术初步探索大鼠死后角膜组织随死亡时间(Postmortem Interval, PMI)的化学变化规律及影响因素。方法 取 65 只 SD 大鼠,于死后 0、6、12、24、36、48 h提取角膜样本并采集 ATR-FTIR 光谱,观察 48 h 内大鼠角膜主要吸收峰峰位、峰形、吸光度及其比值的变化,比较不同性别、眼别、角膜组织离体与在体吸光度比值差异。结果 随 PMI 延长,不同性别、眼别、组织离体与在体的大鼠死后各时间点角膜主要吸收峰吸光度比值均无显著性差异(P ﹥ 0.05)。大鼠死后 48 h 内角膜 FTIR 光谱主要吸收峰峰位及峰形随 PMI 变化无明显变化,吸光度比值 A1339/A1239(r=0.863)、A1453/ A1239(r=0.824)与 PMI呈显著正相关,最佳拟合曲线为三次方曲线,回归方程分别为:y=0.778-0.001x-0.000001403x^(3)(R^(2)=0.786);y= 0.957-0.003x- 0.000003419x^(3)(R^(2)=0.718)。结论 应用 FTIR 光谱技术检测死后角膜光谱改变对 PMI 推断有一定的应用价值,眼别、性别、组织离体等因素的影响可忽略不计。Objective Attenuated total reflection-fourier transform infrared(ATR-FTIR) spectroscopy was utlized to explore the chemical changes in postmortem corneal tissue of rats with the extension of postmortem interval(PMI) as well as influencing factors. Methods 65 SD rats were taken, corneal samples at 0, 6, 12, 24, 36, 48 h postmortem were collected, as well as their ATR-FTIR spectra. The changes of ATR-FTIR characteristics within 48 h were observed, including the peak position, peak shape, absorbance and ratio of the main absorption peaks. The differences of absorbance ratios between gender, ocular category, and tissue in vitro and vivo were compare. Results As PMI was prolonged, there was no significant difference between the ratio of the main FTIR absorbances of different gender, ocular category, and corneal tissue in vitro and vivo at each time postmortem point(P ﹥ 0.05). The peak position did not change significantly with the changes of PMI within 48h postmortem of the rats. So did the shape of the main absorption peaks of the corneal FTIR spectrum. The absorbance ratios at A1339/A1239(r = 0.863), A1453/A1239(r = 0.824) showed a significant positive correlation with PMI, and the best fitting curves were cubic, with the regression equations: y = 0.778-0.001x-0.000001403x^(3)(R^(2) = 0.786);y = 0.957-0.003x-0.000003419x3(R^(2) = 0.718). Conclusions The application of FTIR spectroscopy to detect post-mortem corneal spectroscopy changes has the application value to PMI inference, and the influences of factors, such as gender, ocular category and tissue in vitro, is not significant.

关 键 词：法医病理学 ATR-FTIR 死亡时间 角膜 影响因素 

分 类 号：R349.69[医药卫生—基础医学]