4种中药组方对猪致病性大肠杆菌治疗效果的研究  

Therapeutic Effects of Four Traditional Chinese Compounds on the Disease Infected by Swine Pathogenic Escherichia coli

作　　者：崔曦予 张加力 苏家兴[1,2] 刘潼 CUI Xiyu;ZHANG Jiali;SU Jiaxing;LIU Tong(Jinong Hefeng Animal Hospital,Changchun 130022,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉农禾丰动物医院,长春130022 [2]吉林农业大学动物科学技术学院,长春130118

出　　处：《经济动物学报》2022年第3期197-203,共7页Journal of Economic Animal

基　　金：长春市畜禽无抗养殖技术推广项目(20200000004)。

摘　　要：为了获得有较好疗效的治疗仔猪大肠杆菌病的中药组方,采用中兽医学理论拟定了 4个中药组方.利用建立的人工感染小鼠大肠杆菌病病理模型,分别将4个组方(以生药计)按50,20,10 g/kg的剂量灌服治疗组小鼠,感染组和空白组均灌服无菌生理盐水,持续观察并记录.7 d后测量小鼠血常规,用酶联免疫吸附法测量小鼠血液中免疫球蛋白IgG、IgA及炎性因子IL-1β、CRP的含量.结果表明:与空白组相比,治疗组小鼠血液中与细菌感染引起炎症反应有关的白细胞总数和中性粒细胞水平升高,但低于感染组.与空白组相比,治疗组小鼠血清中IgG、IgA水平降低,但高于感染组;与空白组相比,治疗组小鼠血清中IL-1β、CRP水平升高,但低于感染组.说明4种中药组方均能使治疗组小鼠血液中主要炎性细胞数量下降,免疫球蛋白含量升高,达到了缓解炎症反应和提高动物免疫力的作用.测得50 g/kg剂量的B1组方有较好的效果,为有效治疗仔猪大肠杆菌病提供了理论基础.In order to obtain a better curative effect of traditional Chinese medicine prescription on Escherichia coli disease in piglets,four traditional Chinese medicine prescriptions were formulated according to the theory of traditional Chinese veterinary medicine.Using the established E.coli artificially infected mice model,the 4 prescriptions(based on crude drugs)were administered to the mice in the treatment groups at doses of 50,20,and 10 g/kg,respectively,and the infected group and the control group were given sterile saline.After 7 days,the blood routine of mice was measured,and the contents of immunoglobulin IgG,IgA,inflammatory factors IL-1βand CRP in the blood of mice were measured by enzyme-linked immunosorbent assay.The results showed that compared with the control group,the total number of white blood cells and neutrophil levels related to inflammatory response caused by bacterial infection in the blood of mice in the treatment group were increased,but lower than those in the infection group.Compared with the control group,the levels of IgG and IgA in the serum of mice in the treatment group were lower,but higher than those in the infection group.Compared with the control group,the levels of IL-1βand CRP in the serum of mice in the treatment group were increased,but lower than those in the infection group.It indicated that the four TCM prescriptions could reduce the number of major inflammatory cells in the blood of mice,increase the content of immunoglobulin,alleviate the inflammatory response,and improve the immunity of animals.And 50 g/kg dose of B1 prescription has a good curative effect,which will provide a theoretical basis for the effective treatment of piglet colibacillosis.

关 键 词：大肠杆菌病 中药 炎性因子 抗体 治疗 

分 类 号：S853.7[农业科学—临床兽医学]