^(60)Co-γ辐照对板层人工角膜中指示病毒灭活效果的研究  

Inactivation efficacy of indicator virus in keratoprosthesis by ^(60)Co-γ ray irradiation

作　　者：岳广智[1] 杨立宏[1] 徐宏山[1] 刘欣玉[1] 李玉华[1] YUE Guang-zhi;YANG Li-hong;XU Hong-shan;LIU Xin-yu;LI Yu-hua(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《中国消毒学杂志》2022年第8期561-563,共3页Chinese Journal of Disinfection

摘　　要：目的研究^(60)Co⁃γ辐照对板层人工角膜中模拟污染指示病毒的灭活效果。方法板层人工角膜放入经预先精确测定病毒滴度的辛德毕斯病毒、脑心肌炎病毒和猪细小病毒中,4℃浸泡角膜8 h吸附病毒。经-0.75 kPa减压干燥6 h,进行5~25 kGy不同剂量的^(60)Co⁃γ辐照。辐照后每只角膜用稀释液浸泡释放病毒(4℃,5 h)、剪碎、研磨离心取上清,用蚀斑法或微量细胞病变法分别测定3种病毒的残余病毒滴度,验证^(60)Co⁃γ辐照对3种病毒的灭活效果。结果经5~25 kGy ^(60)Co⁃γ辐照,辛德毕斯病毒滴度下降对数值≥5.32~5.41 lg pfu/mL,脑心肌炎病毒下降对数值≥4.68~5.00 lg TCID_(50)/0.1 mL和猪细小病毒下降对数值≥4.06~4.44 lg TCID_(50)/0.1 mL。结论^(60)Co⁃γ辐照对板层人工角膜中的脂包膜病毒和非脂包膜病毒均有较好的灭活效果。Objective To verify the inactivation efficacy of indicator virus in keratoprostheses by ^(60)Co⁃γ ray irradiation.Method Keratoprostheses were soaked in Sindbis virus,encephalomyocarditis virus and porcine parvovirus respectively at 4℃ for 8 hours to absorb virus.Then,the simulated production process was conducted under the conditions of temperature:18.3℃,-0.75 kPa for 6 hours drying,followed by 5-25 kGy doses of ^(60)Co⁃γ ray irradiation.After irradiation,each Keratoprosthesis was soaked in 0.6 mL diluent at 4℃ for 5 hours to release the virus.Then the keratoprostheses were cut up,ground and centrifuged to collect the supernantants.The titer of Sindbis virus was determined by plaque formation assay,while those of EMCV and PPV by micro-CPE to verify the inactivation effect of ^(60)Co⁃γ ray irradiation on three viruses.Results After drying and irradiation of 5-25 kGy ^(60)Co⁃γ ray,the titers of Sindbis virus,EMCV and PPV decreased>5.32-5.41 lg pfu/mL,≥4.68-5.00 lg TCID_(50)/0.1 mL and≥4.06-4.44 lg TCID_(50)/0.1 mL respectively.Conclusion The ^(60)Co⁃γ ray at a dosage of 25 kGy had good inactivation effect on both lipid-enveloped virus and non-lipid-enveloped virus in keratoprostheses.

关 键 词：^(60)Co⁃γ辐照 板层人工角膜 辛德毕斯病毒 脑心肌炎病毒 猪细小病毒 病毒灭活 

分 类 号：R187.4[医药卫生—流行病学]