青海省大麦黄矮病毒GPV株系小麦分离物基因组克隆及序列特征分析  

Genomic cloning and sequence analysis of an isolate of barley yellow dwarf virus GPV strain from wheat in Qinghai Province

作　　者：乔世英 罗海林 季英华[2] 侯璐 姚强 闫佳会 郭青云 Qiao Shiying;Luo Hailin;Ji Yinghua;Hou Lu;Yao Qiang;Yan Jiahui;Guo Qingyun(Scientific Observing and Experimental Station of Crop Pest of Ministry of Agriculture and Rural Affairs in Xining,Key Laboratory of Agricultural Integrated Pest Management,Academy of Agriculture and Forestry Science,Qinghai University,Xining 810016,Qinghai Province,China;Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base,Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu Province,China)

机构地区：[1]青海大学农林科学院,青海省农业有害生物综合治理重点实验室,农业农村部西宁作物有害生物科学观测实验站,西宁810016 [2]江苏省农业科学院植物保护研究所,江苏省食品质量安全重点实验室-省部共建国家重点实验室培养基地,南京210014

出　　处：《植物保护学报》2022年第4期1032-1038,共7页Journal of Plant Protection

基　　金：青海省应用基础研究计划(2022-ZJ-722)。

摘　　要：为有效防控大麦黄矮病毒(barley yellow dwarf virus,BYDV)病,于2019年自青海省循化县小麦田采集疑似感病病叶,并对其进行反转录PCR(reverse transcription PCR,RT-PCR)检测,并克隆得到BYDV-GPV株系全基因组序列,对克隆的全基因组序列进行系统发育树分析和碱基突变分析。结果显示,在采集样品中检测到BYDV-GPV株系,克隆得到了BYDV-GPV小麦分离物基因组,其序列全长为5676 bp,包含3个非编码区和6个开放阅读框,编码6个蛋白,与BYDV-GPV核苷酸序列的一致性高达98.17%,并聚在一个分支上,表明该分离物为BYDV-GPV。获得的青海BYDV-GPV序列在3′UTR出现碱基突变,多了4个碱基。In order to effectively prevent and control barley yellow dwarf virus(BYDV)disease,diseased wheat leaf samples with yellow dwarf disease were collected from Xunhua,Qinghai Province in 2019.Positive band was obtained from leaf samples in reverse transcription PCR(RT-PCR)detection using BYDV specific primers,the BYDV-GPV whole genome sequence was then cloned,and the sequence was analyzed.The results revealed that the genome of the BYDV-GPV isolate is 5676 bp in length,contains three untranslated regions and six open reading frames,coding six proteins.The sequence is up to 98.17%identical with the previously reported GPV sequence from Czech,and they were clustered into the same clade.These results indicated that the virus associated with wheat yellow dwarf disease in Qinghai Province was BYDV-GPV.The results of base mutation analysis showed that the Qinghai BYDV-GPV sequence had an insertion of four bases in the 3’UTR compared with the BYDV-GPV isolate from Czech.

关 键 词：青海省 小麦 BYDV-GPV 全基因组 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]