SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites  

作　　者：Li Yang Te Liang Lane M.Pierson Hongye Wang Jesse K.Fletcher Shu Wang Duran Bao Lii Zhang Zhen Huang Wenshu Zheng Xiaomei Zhang Heewon Park Yuwen Li James E.Robinson Amy K.Fechan Christopher J.Lyon Jing Cao Lisa A.Morici Chenzhong Li Chad J.Roy Xiaobo Yu Tony Hu 

机构地区：[1]Center for Cllular and Molecular Diagnostics,Tulane University Schoo of Medicine,1430 Tulane Ave,New Orleans,LA 70112,USA [2]Department of Biochemisty and Molecular Biology,Tulane University School of Medicine,1430 Tulane Ave,New Orleans,LA 70112,USA [3]Beijing Key Laboratory for Forest Pest Control,Bejjing Foresiry Uniersily,Beijing 10083.China [4]State Key Laboratory of Proteommics,Beijing Protcome Resarch Center,Nattional Center for Protein Sciences-Beiing(PHOENIX Center),Beijing Institute of Lijfeomics,Beijing 102206,China [5]Hayward Genetis Cener,Department of Pediatrics,Tulane Universily School of Medicine,1430 Tulane Ave,New Orleans,LA 70112,USA [6]Department of Pediatrics,Tulane Uhriversity School of Medicine,1430 Ttulane Ave,New Orleans,LA 70112,USA [7]Infectious Disease Depariment,Ochsner Clinic Foundation,New Orleans,LA 70121,USA [8]Universty of Texas Souhwestern Medical Cener,5323 Harry Himes Blvd,Dallas,TX 75390,USA [9]Department of Microbiology Imruolog Tulane Untiversity School of Medicine,1430 Tulane Ave,New Orleans,LA 70112,USA [10]Division of Microbiology,Tulane National Primate Research Center,18703 Three Rivers Road,Covington,LA 70433,USA

出　　处：《Research》2022年第2期71-84,共14页研究（英文）

基　　金：supported by the Department of Defense(grant number W8IxwH19i0926);National Institute of Allergy and Infectious Diseases contract(grant number_HHSN2722017000331);National Institute of Child Health and Human Development grant(grant numbers R01HD090927 and R01HDi03511);National Center for Research Resources and the Ofice of Research Infrastructure Programs(grant numbers OD011104).

摘　　要：Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed peptide microarrays to map linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and vaccination.LPEs detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned with reported neutralizing antibody binding sies.Similar LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stimulus,where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function.Vaccine-specifc LPEs tended to map to sites known or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein.Mapping LPEs to regions of known functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.

关 键 词：vaccine ANTIBODY LIKELY 

分 类 号：R563.1[医药卫生—呼吸系统] R186[医药卫生—内科学]