黑素皮质激素-1受体基因多态性与创伤后脓毒症易感性的关联  

Association between melanocortin-1 receptor gene polymorphism and susceptibility to post-traumatic sepsis

作　　者：杨凯 靳晶[1] 邵瑞娜 单天琪 彭玉芳 YANG Kai;JIN Jing;SHAO Rui-na;SHAN Tian-qi;PENG Yu-fang(Baoding First Central Hospital,Baoding,Hebei 071000,China;不详)

机构地区：[1]保定市第一中心医院院前急救科,河北保定071000 [2]北京儿童医院保定医院重症监护室,河北保定071000

出　　处：《中华医院感染学杂志》2022年第3期422-425,共4页Chinese Journal of Nosocomiology

基　　金：河北省卫健委重点研究室支撑基金资助项目(冀中医药[2014]28号-14);河北省卫健委重点科技研究计划基金资助项目(20181485)。

摘　　要：目的 探讨黑素皮质激素-1受体(MC1R)基因多态性与创伤后脓毒症易感性的关联。方法 选择保定市第一中心医院急救科2018年1月—2021年1月连续收治的70例创伤后脓毒症患者为研究组,于同期连续性收集70例创伤后未发生脓毒症患者为对照组。收集患者外周静脉血针对MC1R基因rs885479(c.488G>A)位点设计特异性引物,采用聚合酶链式扩增反应(PCR)扩增MC1R基因目的片段,将扩增产物回收后送公司进行测序,比较研究组、对照组以及研究组不同预后(28 d死亡情况)患者MC1R基因rs885479分布情况。结果 研究组MC1R基因rs885479位点AA基因型、A等位基因频率高于对照组,GG基因型、G等位基因频率低于对照组(P<0.05);多因素Logistic回归分析结果显示,携带MC1R基因rs885479位点AA基因型、A等位基因为创伤后发生脓毒症风险因素,携带GG基因型为保护因素(P<0.05);死亡组MC1R基因rs885479位点AA基因型、A等位基因频率高于存活组,GG基因型、G等位基因频率低于对照组,差异具有统计学意义(P<0.05)。结论 MC1R基因rs885479位点AA基因型显著增加本地区人群创伤后脓毒症发生率,且与患者临床预后有关。OBJECTIVE To explore the association between Melanocortin-1 receptor(MC1 R) gene polymorphism and the susceptibility to post-traumatic sepsis. METHODS The 70 patients with post-traumatic sepsis admitted to the emergency department of Baoding First General Hospital from Jan 2018 to Jan 2021 were selected as the study group, and 70 patients without post-traumatic sepsis during the same period were continuously collected as the control group. Peripheral venous blood of patients were collected, and specific primers of MC1 R gene rs885479(c.488 G>A) site were designed. The target fragment of MC1 R gene was amplified by polymerase chain amplification reaction(PCR). The amplified products were recovered and sent to the company for sequencing. The distribution of MC1 R gene rs885479 in the study group, the control group, and the study group with different prognosis(28-day mortality) was compared. RESULTS The frequency of AA genotype and A allele at rs885479 locus of MC1 R gene in significantly higher than that of the control group, while the frequency of GG genotype and G allele was significantly lower than that of the control group. The results of multivariate logistic analysis showed that carrying with AA genotype and A allele of MC1 R gene at rs885479 was risk factors for sepsis after trauma, and genotype carrying GG was a protective factor(P<0.05). The frequency of AA genotype and A allele at MC1 R gene at rs885479 in the death group was significantly higher than that of the survival group, and the frequency of GG genotype and G allele was significantly lower than that of the control group, and the difference was statistically significant(P<0.05). CONCLUSION The AA genotype of MC1 R gene at rs885479 locus significantly increased the incidence of post-traumatic sepsis in the population in this area, which was related to the clinical prognosis of patients.

关 键 词：黑素皮质激素-1受体 基因多态性 脓毒症 创伤 预后 

分 类 号：R631[医药卫生—外科学]