胎膜早破并发宫内感染产妇LncRNA CRNDE和miR-182表达及其与新生儿脑损伤的关系  

The expression of LncRNA CRNDE and miR-182 in pregnant women with premature rupture of membranes and intrauterine infection and their relationship with neonatal brain damage

作　　者：赵宝君[1] 张卫星[1] 王平[1] 刘芳[1] 刘雪琴[1] ZHAO Bao-jun;ZHANG Wei-xing;WANG Ping;LIU Fang;LIU Xue-qin(Xinxiang Central Hospital(The Fourth Clinical College Xinxiang Medical University),Xinxciang,Henan 453000,China)

机构地区：[1]新乡市中心医院(新乡医学院第四临床医院)新生儿重症监护病房,河南新乡453000

出　　处：《中华医院感染学杂志》2022年第3期466-470,共5页Chinese Journal of Nosocomiology

基　　金：河南省医学科技攻关计划基金资助项目(LHGJ20191319)。

摘　　要：目的 探讨胎膜早破(PROM)并宫内感染产妇长链非编码RNA(LncRNA)CRNDE和miR-182表达及其与新生儿脑损伤的关系。方法 选择新乡市中心医院2018年3月—2020年5月新生儿重症监护病房进行分娩的PROM合并宫内感染产妇113例为研究对象,根据纳入产妇所生产新生儿是否发生脑损伤将其分为脑损伤组42例和非脑损伤组71例。所有产妇均在分娩当天抽取外周静脉血,提取外周血RNA,采用实时荧光定量聚合酶链式扩增反应检测LncRNA CRNDE和miR-182表达。分析LncRNA CRNDE和miR-182水平与新生儿脑损伤、脑损伤严重程度的关系,受试者工作特征曲线(ROC)分析其预测价值。结果 脑损伤组LncRNA CRNDE、miR-182水平高于非脑损伤组(P<0.05);脑损伤严重组LncRNA CRNDE、miR-182水平高于脑损伤中等组及脑损伤较轻组,脑损伤中等组LncRNA CRNDE、miR-182水平高于脑损伤较轻组,差异均具有统计学意义(P<0.05);ROC分析结果显示,产后当天产妇外周血LncRNA CRNDE、miR-182对于新生儿脑损伤预测的曲线下面积分别为0.755、0.742,均具有统计学意义(P<0.05)。结论 产后外周血LncRNA CRNDE和miR-182水平对于预测新生儿脑损伤发生及评估其严重程度有一定临床价值。OBJECTIVE To explore the expression of long non-coding RNA CRNDE and miR-182 in women with premature rupture of membranes and intrauterine infection and their relationship with neonatal brain damage. METHODS A total of 113 cases of PROM and intrauterine infection women who gave birth in the neonatal intensive care unit of Xinxiang Central Hospital from Mar 2018 to May 2020 were selected as the research subjects and divided into 42 cases of brain injury group and 71 cases pf the non-brain injury group according to whether the newborns delivered by the included mothers had brain damage. The peripheral blood samples was collected from all parturients on the day of delivery, and RNA was extracted from peripheral blood. Real-time fluorescent quantitative polymerase chain amplification reaction was used to detect the expression of LncRNA, CRNDE and miR-182. The relationship between LncRNA CRNDE and miR-182 levels and the severity of neonatal brain injury and brain injury was analyzed, and ROC was used to analyze the predictive value. RESULTS The levels of LncRNA CRNDE and miR-182 in the brain injury group were significantly higher than those in the non-brain injury group(P>0.05);The levels of LncRNA CRNDE and miR-182 in the severe brain injury group were significantly higher than those in the moderate brain injury group and the mild brain injury group. The levels of LncRNA CRNDE and miR-182 in the moderate brain injury group were significantly higher than those in the mild brain injury group(P<0.05);ROC analysis results showed that the area under the curve of LncRNA CRNDE and miR-182 in maternal peripheral blood for the prediction of neonatal brain injury on the postpartum day was 0.755 and 0.742, respectively, which were significantly different(P<0.05).CONCLUSION The levels of long-chain non-coding RNA CRNDE and miR-182 in postpartum peripheral blood had certain clinical value for predicting the occurrence of neonatal brain injury and assessing its severity.

关 键 词：长链非编码RNA miR-182 胎膜早破 宫内感染 新生儿脑损伤 

分 类 号：R714.433[医药卫生—妇产科学]